ArticleProceedings of the National Academy of Sciences of the United States of America2015
Spatiotemporally dynamic, cell-type-dependent premeiotic and meiotic phasiRNAs in maize anthers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers.
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176 citing papers in PubMed, 341 citations in OpenAlex.
- Presence and function of small RNAs during plant reproduction.RNA biology · 2026Review
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- RNA regulation in plants.Science China. Life sciences · 2026Review
- The annotated blueprint: integrated functional genomic resources for a model tetraploid wheat Triticum turgidum cv Kronos.The New phytologist · 2026Article
- MicroRNA networks in rice seeds: unveiling key regulators of development, germination, and rice resilience under climate changes.Journal of experimental botany · 2026Review
- Reproductive phasiRNAs are the piRNAs of plants.Trends in genetics : TIG · 2026Review
- Long-distance transport of siRNAs with functional roles in pollen development.Nature plants · 2026Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- The Annotated Blueprint: Integrated Functional Genomic Resources for a model Tetraploid WheatbioRxiv : the preprint server for biology · 2025Article
- Distinct classes of 21- and 24-nt phasiRNAs suggests diverse mechanisms of biogenesis and function in rice anther development.The plant genome · 2025Article
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- Spatiotemporal regulation of target mRNA cleavage by 21-nt phasiRNAs in maize anthers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Non-conserved microRNAs and their roles in plants: the case for legumes.Plant biology (Stuttgart, Germany) · 2025Review
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- Unraveling the Multilayered Regulatory Networks of miRNAs and PhasiRNAs inPlants (Basel, Switzerland) · 2025Article
- Sexual reproduction in land plants: an evolutionary perspective.Plant reproduction · 2025Review
- Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducingGenome research · 2025Article
116 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maize anthers, the male reproductive floral organs, express two classes of phased small-interfering RNAs (phasiRNAs). PhasiRNA precursors are transcribed by RNA polymerase II and map to low-copy, intergenic regions similar to PIWI-interacting RNAs (piRNAs) in mammalian testis. From 10 sequential cohorts of staged maize anthers plus mature pollen we find that 21-nt phased siRNAs from 463 loci appear abruptly after germinal and initial somatic cell fate specification and then diminish, whereas 24-nt phasiRNAs from 176 loci coordinately accumulate during meiosis and persist as anther somatic cells mature and haploid gametophytes differentiate into pollen. Male-sterile ocl4 anthers defective in epidermal signaling lack 21-nt phasiRNAs. Male-sterile mutants with subepidermal defects--mac1 (excess meiocytes), ms23 (defective pretapetal cells), and msca1 (no normal soma or meiocytes)--lack 24-nt phasiRNAs. ameiotic1 mutants (normal soma, no meiosis) accumulate both 21-nt and 24-nt phasiRNAs, ruling out meiotic cells as a source or regulator of phasiRNA biogenesis. By in situ hybridization, miR2118 triggers of 21-nt phasiRNA biogenesis localize to epidermis; however, 21-PHAS precursors and 21-nt phasiRNAs are abundant subepidermally. The miR2275 trigger, 24-PHAS precursors, and 24-nt phasiRNAs all accumulate preferentially in tapetum and meiocytes. Therefore, each phasiRNA type exhibits independent spatiotemporal regulation with 21-nt premeiotic phasiRNAs dependent on epidermal and 24-nt meiotic phasiRNAs dependent on tapetal cell differentiation. Maize phasiRNAs and mammalian piRNAs illustrate putative convergent evolution of small RNAs in male reproduction.
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