ArticleProceedings of the National Academy of Sciences of the United States of America2015
Rapid selection against arbovirus-induced apoptosis during infection of a mosquito vector.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Who cites it
43 citing papers in PubMed, 85 citations in OpenAlex.
- Unlocking the Black Box: The Molecular Dialogue Between ASFV and Its Tick Host.Pathogens (Basel, Switzerland) · 2026Review
- Plant Virus-Induced Inheritable Apoptosis Drives Reproductive Costs in Female Insect Vectors to Balance Viral Biparental Transmission.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Alteration of mitochondrial function in arthropods during arboviruses infection: a review of the literature.Frontiers in physiology · 2025Review
- Article
- The midgut epithelium of mosquitoes adjusts cell proliferation and endoreplication to respond to physiological challenges.BMC biology · 2024Article
- Wolbachia-mediated resistance to Zika virus infection in Aedes aegypti is dominated by diverse transcriptional regulation and weak evolutionary pressures.PLoS neglected tropical diseases · 2023Article
- Article
- Hypoxia-induced tracheal elasticity in vector beetle facilitates the loading of pinewood nematode.eLife · 2023Article
- The JAK-STAT pathway promotes persistent viral infection by activating apoptosis in insect vectors.PLoS pathogens · 2023Article
- Insect-Specific Chimeric Viruses Potentiated Antiviral Responses and Inhibited Pathogenic Alphavirus Growth in Mosquito Cells.Microbiology spectrum · 2023Article
- Factors Affecting Arbovirus Midgut Escape in Mosquitoes.Pathogens (Basel, Switzerland) · 2023Review
- The Role of Mosquito Hemocytes in Viral Infections.Viruses · 2022Review
- Article
- The nucleocapsid protein of rice stripe virus in cell nuclei of vector insect regulates viral replication.Protein & cell · 2022Article
- Investigation of Biological Factors Contributing to Individual Variation in Viral Titer after Oral Infection ofViruses · 2022Article
- Isobaric tags for relative and absolute quantification-based proteomic analysis of host-pathogen protein interactions in the midgut ofFrontiers in microbiology · 2022Article
- Infection of Aedes aegypti Mosquitoes with Midgut-Attenuated Sindbis Virus Reduces, but Does Not Eliminate, Disseminated Infection.Journal of virology · 2021Article
- Cell-Type Apoptosis in Lung during SARS-CoV-2 Infection.Pathogens (Basel, Switzerland) · 2021Article
- Clustered rapid induction of apoptosis limits ZIKV and DENV-2 proliferation in the midguts of Aedes aegypti.Communications biology · 2021Article
- Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Millions of people are infected each year by arboviruses (arthropod-borne viruses) such as chikungunya, dengue, and West Nile viruses, yet for reasons that are largely unknown, only a relatively small number of mosquito species are able to transmit arboviruses. Understanding the complex factors that determine vector competence could facilitate strategies for controlling arbovirus infections. Apoptosis is a potential antiviral defense response that has been shown to be important in other virus-host systems. However, apoptosis is rarely seen in arbovirus-infected mosquito cells, raising questions about its importance as an antiviral defense in mosquitoes. We tested the effect of stimulating apoptosis during arbovirus infection by infecting Aedes aegypti mosquitoes with a Sindbis virus (SINV) clone called MRE/Rpr, in which the MRE-16 strain of SINV was engineered to express the proapoptotic gene reaper from Drosophila. MRE/Rpr exhibited an impaired infection phenotype that included delayed midgut infection, delayed virus replication, and reduced virus accumulation in saliva. Nucleotide sequencing of the reaper insert in virus populations isolated from individual mosquitoes revealed evidence of rapid and strong selection against maintenance of Reaper expression in MRE/Rpr-infected mosquitoes. The impaired phenotype of MRE/Rpr, coupled with the observed negative selection against Reaper expression, indicates that apoptosis is a powerful defense against arbovirus infection in mosquitoes and suggests that arboviruses have evolved mechanisms to avoid stimulating apoptosis in mosquitoes that serve as vectors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.