ArticleThe Journal of biological chemistry2015
Multidomain human peroxidasin 1 is a highly glycosylated and stable homotrimeric high spin ferric peroxidase.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Validation and performance assessment of a commercial anti-peroxidasin antibody.Histochemistry and cell biology · 2026Article
- Peroxidasin Inhibition by Phloroglucinol and Other Peroxidase Inhibitors.Antioxidants (Basel, Switzerland) · 2023Article
- The role of peroxidasin in solid cancer progression.Biochemical Society transactions · 2023Review
- Measuring peroxidasin activity in live cells using bromide addition for signal amplification.Redox biology · 2022Article
- Uric Acid Reacts with Peroxidasin, Decreases Collagen IV Crosslink, Impairs Human Endothelial Cell Migration and Adhesion.Antioxidants (Basel, Switzerland) · 2022Article
- Air pollution particles hijack peroxidasin to disrupt immunosurveillance and promote lung cancer.eLife · 2022Article
- Mammalian peroxidasin (PXDN): From physiology to pathology.Free radical biology & medicine · 2022Review
- Characterization of the Proprotein Convertase-Mediated Processing of Peroxidasin and Peroxidasin-like Protein.Antioxidants (Basel, Switzerland) · 2021Article
- Inhibition of Myeloperoxidase.Handbook of experimental pharmacology · 2021Article
- Role of Extracellular Matrix in Pathophysiology of Patent Ductus Arteriosus: Emphasis on Vascular Remodeling.International journal of molecular sciences · 2020Review
- Peroxidase Activity of Human Hemoproteins: Keeping the Fire under Control.Molecules (Basel, Switzerland) · 2018Review
- Role of Hypohalous Acids in Basement Membrane Homeostasis.Antioxidants & redox signaling · 2017Review
- Pre-steady-state Kinetics Reveal the Substrate Specificity and Mechanism of Halide Oxidation of Truncated Human Peroxidasin 1.The Journal of biological chemistry · 2017Article
- Proprotein Convertase Processing Enhances Peroxidasin Activity to Reinforce Collagen IV.The Journal of biological chemistry · 2016Article
- The Ancient Immunoglobulin Domains of Peroxidasin Are Required to Form Sulfilimine Cross-links in Collagen IV.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
Human peroxidasin 1 (hsPxd01) is a multidomain heme peroxidase that uses bromide as a cofactor for the formation of sulfilimine cross-links. The latter confers critical structural reinforcement to collagen IV scaffolds. Here, hsPxd01 and various truncated variants lacking nonenzymatic domains were recombinantly expressed in HEK cell lines. The N-glycosylation site occupancy and disulfide pattern, the oligomeric structure, and unfolding pathway are reported. The homotrimeric iron protein contains a covalently bound ferric high spin heme per subunit with a standard reduction potential of the Fe(III)/Fe(II) couple of -233 ± 5 mV at pH 7.0. Despite sequence homology at the active site and biophysical properties similar to human peroxidases, the catalytic efficiency of bromide oxidation (kcat/KM(app)) of full-length hsPxd01 is rather low but increased upon truncation. This is discussed with respect to its structure and proposed biosynthetic function in collagen IV cross-linking.
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Registered trials
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