ArticleInfection and immunity2015
The IbeA invasin of adherent-invasive Escherichia coli mediates interaction with intestinal epithelia and macrophages.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 78 citations in OpenAlex.
- Review
- EruA, a Regulator of Adherent-InvasiveBiomolecules · 2026Article
- Research Progress on perinatalFrontiers in microbiology · 2026Review
- Persistence mechanisms of Crohn's disease-associated adherent invasiveGut microbes · 2025Article
- Pathogenesis of adherent-invasiveGut microbes · 2025Article
- The assembly of a hybrid type IV secretion system by a Crohn's disease-associated Escherichia coli strain.Nature communications · 2025Article
- Genetic and Microbial Analysis of Invasiveness for Escherichia coli Strains Associated With Inflammatory Bowel Disease.Cellular and molecular gastroenterology and hepatology · 2025Article
- Persistence phenotype of adherent-invasiveMicrobial cell (Graz, Austria) · 2025Article
- Exploring the relationship betweenComputational and structural biotechnology journal · 2024Review
- The Role of Propionate-Induced Rearrangement of Membrane Proteins in the Formation of the Virulent Phenotype of Crohn's Disease-Associated Adherent-InvasiveInternational journal of molecular sciences · 2024Article
- Contribution of Toxin-Antitoxin Systems to Adherent-InvasiveMicroorganisms · 2024Review
- Molecular typing and virulence characteristics of Escherichia coli strains isolated from hospital and community acquired urinary tract infections.Molecular biology reports · 2024Article
- Colonization of larval zebrafish (mSphere · 2023Article
- Characterizing the Pathogenic Potential of Crohn's Disease-Associated Adherent-InvasiveEcoSal Plus · 2023Review
- Article
- Battle royale: Immune response on biofilms - host-pathogen interactions.Current research in immunology · 2023Review
- Article
- Characterization of Adherent-InvasiveInternational journal of molecular sciences · 2022Article
- Mucolytic bacteria license pathobionts to acquire host-derived nutrients during dietary nutrient restriction.Cell reports · 2022Article
- Evaluation of the Pathogenic Potential ofMicroorganisms · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Adherent-invasive Escherichia coli (AIEC) pathogroup isolates are a group of isolates from the intestinal mucosa of Crohn's disease patients that can invade intestinal epithelial cells (IECs) or macrophages and survive and/or replicate within. We have identified the ibeA gene in the genome of AIEC strain NRG857c and report the contribution of IbeA to the interaction of AIEC with IECs and macrophages and colonization of the mouse intestine. An ibeA deletion mutant strain (NRG857cΔibeA) was constructed, and the in vitro effect on AIEC adhesion and invasion of nonpolarized and polarized Caco-2 cells, the adhesion and transcytosis of M-like cells, the intracellular survival in THP-1 macrophages, and the contribution to intestinal colonization of the CD-1 murine model of infection were evaluated. A significant reduction in invasion was observed with the ibeA mutant in Caco-2 and M-like cells, whereas adhesion was not affected. Complementation of the mutant reestablished Caco-2 invasive phenotype to wild-type levels. Reduction in invasion did not significantly affect transcytosis through M-like cells at early time points. The absence of ibeA significantly affected AIEC intramacrophage survival up to 24 h postinfection. No significant changes associated with IbeA were found in AIEC colonization across the murine gastrointestinal tract, but a slight reduction of gamma interferon was observed in the ceca of mice infected with the ibeA mutant. In addition, a decrease in the pathology scores was observed in the ilea and ceca of mice infected with the ibeA mutant. Our data support the function of IbeA in the AIEC invasion process, macrophage survival, and inflammatory response in the murine intestine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.