ArticleInfection and immunity2015
The CpxRA two-component system is essential for Citrobacter rodentium virulence.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 37 citations in OpenAlex.
- O‑Island 28 encodes a type I secretion and RTX adhesion system regulated by RstA and required for early EHEC O157:H7 adherence.Gut microbes · 2026Article
- The divergentMicrobiology (Reading, England) · 2026Article
- Dynamic response by commensal E. coli two-component system Cpx contributes to C. elegans development.BMC biology · 2026Article
- The CpxR response regulator mediates the virulence ofMicrobiology spectrum · 2026Article
- Bacteriophage Mu contamination impacts interbacterial competition.Infection and immunity · 2025Article
- Antibiotic-induced stress responses in Gram-negative bacteria and their role in antibiotic resistance.The Journal of antimicrobial chemotherapy · 2025Review
- Regulatory role of the Cpx ESR in bacterial behaviours.Virulence · 2024Review
- The CpxAR signaling system confers a fitness advantage for flea gut colonization by the plague bacillus.Journal of bacteriology · 2024Article
- Both pathogen and host dynamically adapt pH responses along the intestinal tract during enteric bacterial infection.PLoS biology · 2024Article
- The CpxRA two-component system of adherent and invasiveInfection and immunity · 2024Article
- Systematic Identification of CpxRA-Regulated Genes and Their Roles in Escherichia coli Stress Response.mSystems · 2022Article
- Simulated Colonic Fluid Replicates theInfection and immunity · 2022Article
- Citrobacter rodentium(ϕStx2dact), a murine infection model for enterohemorrhagic Escherichia coli.Current opinion in microbiology · 2022Review
- The Serotonin Neurotransmitter Modulates Virulence of Enteric Pathogens.Cell host & microbe · 2020Article
- The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.Virulence · 2019Article
- Article
- The CpxR regulates type VI secretion system 2 expression and facilitates the interbacterial competition activity and virulence of avian pathogenic Escherichia coli.Veterinary research · 2019Article
- Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2019Review
- Successful Host Adaptation of IncK2 Plasmids.Frontiers in microbiology · 2019Article
- The VraSR regulatory system contributes to virulence in Streptococcus suis via resistance to innate immune defenses.Virulence · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Citrobacter rodentium is a murine intestinal pathogen used as a model for the foodborne human pathogens enterohemorrhagic Escherichia coli and enteropathogenic E. coli. During infection, these pathogens use two-component signal transduction systems to detect and adapt to changing environmental conditions. In E. coli, the CpxRA two-component signal transduction system responds to envelope stress by modulating the expression of a myriad of genes. Quantitative real-time PCR showed that cpxRA was expressed in the colon of C57BL/6J mice infected with C. rodentium. To determine whether CpxRA plays a role during C. rodentium infection, a cpxRA deletion strain was generated and found to have a colonization defect during infection. This defect was independent of an altered growth rate or a defective type III secretion system, and single-copy chromosomal complementation of cpxRA restored virulence. The C. rodentium strains were then tested in C3H/HeJ mice, a lethal intestinal infection model. Mice infected with the ΔcpxRA strain survived infection, whereas mice infected with the wild-type or complemented strains succumbed to infection. Furthermore, we found that the cpxRA expression level was higher during early infection than at a later time point. Taken together, these data demonstrate that the CpxRA two-component signal transduction system is essential for the in vivo virulence of C. rodentium. In addition, these data suggest that fine-tuned cpxRA expression is important for infection. This is the first study that identifies a C. rodentium two-component transduction system required for pathogenesis. This study further indicates that CpxRA is an interesting target for therapeutics against enteric pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.