ArticleNucleic acids research2015
Gene-specific cell labeling using MiMIC transposons.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
55 citing papers in PubMed, 92 citations in OpenAlex.
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- Hierarchical diversification of instinctual behavior neurons by lineage, birth order, and sex.bioRxiv : the preprint server for biology · 2025Article
- Perception and Longevity Control in Invertebrate Model Organisms-A Mini-Review of Recent Advances.Biomolecules · 2025Review
- Serotonin acts through multiple cellular targets during an olfactory critical period.iScience · 2024Article
- Constitutive and Conditional Epitope Tagging of Endogenous G-Protein-Coupled Receptors inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Hox gene-specific cellular targeting using split intein Trojan exons.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Serotonin acts through multiple cellular targets during an olfactory critical period.bioRxiv : the preprint server for biology · 2024Article
- Expanding theeLife · 2024Article
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- Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network.G3 (Bethesda, Md.) · 2023Article
- Ring neurons in the Drosophila central complex act as a rheostat for sensory modulation of aging.PLoS biology · 2023Article
- Multiplexed Transgenic Selection and Counterselection Strategies to Expedite Genetic Manipulation Workflows Using Drosophila melanogaster.Current protocols · 2023Article
- Serial Recombineering Cloning to Build Selectable and Tagged Genomic P[acman] BAC Clones for Selection Transgenesis and Functional Gene Analysis using Drosophila melanogaster.Current protocols · 2023Article
- Synthetic Assembly DNA Cloning to Build Plasmids for Multiplexed Transgenic Selection, Counterselection or Any Other Genetic Strategies Using Drosophila melanogaster.Current protocols · 2023Article
- Sleep-promoting neurons remodel their response properties to calibrate sleep drive with environmental demands.PLoS biology · 2022Article
- An expanded toolkit foreLife · 2022Article
- Chemoreceptor co-expression ineLife · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Binary expression systems such as GAL4/UAS, LexA/LexAop and QF/QUAS have greatly enhanced the power of Drosophila as a model organism by allowing spatio-temporal manipulation of gene function as well as cell and neural circuit function. Tissue-specific expression of these heterologous transcription factors relies on random transposon integration near enhancers or promoters that drive the binary transcription factor embedded in the transposon. Alternatively, gene-specific promoter elements are directly fused to the binary factor within the transposon followed by random or site-specific integration. However, such insertions do not consistently recapitulate endogenous expression. We used Minos-Mediated Integration Cassette (MiMIC) transposons to convert host loci into reliable gene-specific binary effectors. MiMIC transposons allow recombinase-mediated cassette exchange to modify the transposon content. We developed novel exchange cassettes to convert coding intronic MiMIC insertions into gene-specific binary factor protein-traps. In addition, we expanded the set of binary factor exchange cassettes available for non-coding intronic MiMIC insertions. We show that binary factor conversions of different insertions in the same locus have indistinguishable expression patterns, suggesting that they reliably reflect endogenous gene expression. We show the efficacy and broad applicability of these new tools by dissecting the cellular expression patterns of the Drosophila serotonin receptor gene family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.