ArticleGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association2016
TSPAN8, identified by Escherichia coli ampicillin secretion trap, is associated with cell growth and invasion in gastric cancer.
Article in Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 49 citations in OpenAlex.
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- mir-605-3p prevents liver premetastatic niche formation by inhibiting angiogenesis via decreasing exosomal nos3 release in gastric cancer.Cancer cell international · 2024Article
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- TSPAN8 regulates EGFR/AKT pathway to enhance metastasis in gastric cancer.Molecular biology reports · 2023Article
- Peptides disrupting TM4SF3 interaction with AR or AR-V7 block prostate cancer cell proliferation.Endocrine oncology (Bristol, England) · 2023Article
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- Exosomes Promote Pre-Metastatic Niche Formation in Gastric Cancer.Frontiers in oncology · 2021Review
- Tetraspanins: Novel Molecular Regulators of Gastric Cancer.Frontiers in oncology · 2021Review
- Emerging role of exosomes as promising diagnostic tool for cancer.Cancer gene therapy · 2020Review
- TSPAN8 as a Novel Emerging Therapeutic Target in Cancer for Monoclonal Antibody Therapy.Biomolecules · 2020Review
- TSPAN8 serves as a prognostic marker involving Akt/MAPK pathway in nasopharyngeal carcinoma.Annals of translational medicine · 2019Article
- Exosomes: from carcinogenesis and metastasis to diagnosis and treatment of gastric cancer.Cellular and molecular life sciences : CMLS · 2019Review
- Tetraspanin family identified as the central genes detected in gastric cancer using bioinformatics analysis.Molecular medicine reports · 2018Article
- Role of exosomal proteins in cancer diagnosis.Molecular cancer · 2017Review
- Type 1 diabetes cadaveric human pancreata exhibit a unique exocrine tissue proteomic profile.Proteomics · 2016Article
- Fukutin, identified by the Escherichia coli ampicillin secretion trap (CAST) method, participates in tumor progression in gastric cancer.Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2016Article
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9 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundGastric cancer (GC) is one of the most common human cancers. Genes expressed only in cancer tissue, especially on the cell membrane, will be useful biomarkers for cancer diagnosis and therapeutics.
methodsTo identify novel genes encoding transmembrane protein specifically expressed in GC, we generated an Escherichia coli ampicillin secretion trap (CAST) library from diffuse-type GC cell line MKN-45. CAST is a survival-based signal sequence trap method that exploits the ability of mammalian signal sequences to confer ampicillin resistance to a mutant β-lactamase lacking the endogenous signal sequence.
resultsBy sequencing 1,536 colonies, we identified 23 genes encoding the transmembrane protein present in GC. Among these genes, TSPAN8 (also known as CO-029 and TM4SF3) gene, which encodes transmembrane protein tetraspanin 8, was emphasized as a candidate. Immunohistochemical analysis of tetraspanin 8 in human GC tissues revealed that 72 (34 %) of 210 GC cases were positive for tetraspanin 8, and microvessel density was significantly higher in tetraspanin 8-positive GC than in tetraspanin 8-negative GC. Furthermore, univariate and multivariate analyses revealed that tetraspanin 8 expression is an independent prognostic classifier of patients with GC. TSPAN8 knockdown by siRNA reduced the invasion of GC cell line. The reduction of invasiveness was retrieved by the tetraspanin 8-containing exosome.
conclusionThese results suggest that tetraspanin 8 is involved in tumor progression and is an independent prognostic classifier in patients with GC.
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