ArticleScientific reports2015
Whole-mount single molecule FISH method for zebrafish embryo.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Post-transcriptional regulatory networks: The dynamic interplay of RNA-binding proteins.The FEBS journal · 2026Review
- Real-time monitoring of an endogenous Fgf8a gradient attests to its role as a morphogen during zebrafish gastrulation.Development (Cambridge, England) · 2023Article
- Fluid extraction from the left-right organizer uncovers mechanical properties needed for symmetry breaking.eLife · 2023Article
- Article
- CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos.Genome biology · 2023Article
- Combined modelling of mRNA decay dynamics and single-molecule imaging in the Drosophila embryo uncovers a role for P-bodies in 5' to 3' degradation.PLoS biology · 2023Article
- Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.Bioconjugate chemistry · 2022Article
- Article
- The RNA binding protein fragile X mental retardation protein promotes myelin sheath growth.Glia · 2020Article
- Article
- Advanced 3D Analysis and Optimization of Single-Molecule FISH inSmall methods · 2018Article
- Single-cell systems biology: probing the basic unit of information flow.Current opinion in systems biology · 2018Article
- Enzymatic production of single-molecule FISH and RNA capture probes.RNA (New York, N.Y.) · 2017Article
- Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydrogel embedding and clearing.Development (Cambridge, England) · 2016Article
- Memory of cell shape biases stochastic fate decision-making despite mitotic rounding.Nature communications · 2016Article
- Quantitative spatial analysis of transcripts in multinucleate cells using single-molecule FISH.Methods (San Diego, Calif.) · 2016Article
- Extending the dynamic range of transcription factor action by translational regulation.Physical review. E · 2016Article
- A simple and rapid method for combining fluorescent in situ RNA hybridization (FISH) and immunofluorescence in the C. elegans germline.MethodsX · 2016Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noise in gene expression renders cells more adaptable to changing environment by imposing phenotypic and functional heterogeneity on genetically identical individual cells. Hence, quantitative measurement of noise in gene expression is essential for the study of biological processes in cells. Currently, there are two complementary methods for quantitatively measuring noise in gene expression at the single cell level: single molecule FISH (smFISH) and single cell qRT-PCR (or single cell RNA-seq). While smFISH has been developed for culture cells, tissue sections and whole-mount invertebrate organisms, the method has not been reported for whole-mount vertebrate organisms. Here, we report an smFISH method that is suitable for whole-mount zebrafish embryo, a popular vertebrate model organism for the studies of development, physiology and disease. We show the detection of individual transcripts for several cell-type specific and ubiquitously expressed genes at the single cell level in whole-mount zebrafish embryo. We also demonstrate that the method can be adapted to detect two different genes in individual cells simultaneously. The whole-mount smFISH method described in this report is expected to facilitate the study of noise in gene expression and its role in zebrafish, a vertebrate animal model relevant to human biology.
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