ArticleJournal of experimental botany2015
Metabolite fingerprinting of pennycress (Thlaspi arvense L.) embryos to assess active pathways during oil synthesis.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- A pennycress transparent testa 8 knockout mutant has drastic changes in seed coat anatomy and chemical compositions.Journal of experimental botany · 2026Article
- Comparative Metabolomic Profiling of Resistant and SusceptiblePlants (Basel, Switzerland) · 2026Article
- Mapping carbon utilization pathways inmSystems · 2025Article
- Propelling sustainable energy: Multi-omics analysis of pennycress FATTY ACID ELONGATION1 knockout for biofuel production.Plant physiology · 2025Article
- Targeted modulation of pennycress lipid droplet proteins impacts droplet morphology and seed oil content.The Plant journal : for cell and molecular biology · 2024Article
- Proteomic Profiling ofInternational journal of molecular sciences · 2024Article
- 13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri.Journal of experimental botany · 2024Article
- Transcriptomic and lipidomic analysis of the differential pathway contribution to the incorporation of erucic acid to triacylglycerol during Pennycress seed maturation.Frontiers in plant science · 2024Article
- Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement.Plant biotechnology journal · 2023Article
- Research progress on the development of pennycress (Frontiers in plant science · 2023Review
- Genetic and environmental drivers of large-scale epigenetic variation in Thlaspi arvense.PLoS genetics · 2022Article
- Metabolomic Analysis of Wheat Grains afterMetabolites · 2022Article
- Natural variation and improved genome annotation of the emerging biofuel crop field pennycress (Thlaspi arvense).G3 (Bethesda, Md.) · 2022Article
- Effective Mechanisms for Improving Seed Oil Production in Pennycress (Frontiers in plant science · 2022Article
- A lipidomics platform to analyze the fatty acid compositions of non-polar and polar lipid molecular species from plant tissues: Examples from developing seeds and seedlings of pennycress (Frontiers in plant science · 2022Article
- Current Proteomic and Metabolomic Knowledge of Zygotic and Somatic Embryogenesis in Plants.International journal of molecular sciences · 2021Review
- Targeted Metabolic Profiles of the Leaves and Xylem Sap of Two Sugarcane Genotypes Infected with the Vascular Bacterial PathogenMetabolites · 2021Article
- Analyzing Mass Spectrometry Imaging Data ofMetabolites · 2021Article
- Functional analysis of β-ketoacyl-CoA synthase from biofuel feedstock Thlaspi arvense reveals differences in the triacylglycerol biosynthetic pathway among Brassicaceae.Plant molecular biology · 2020Article
- Non-conventional pathways enable pennycress (Thlaspi arvense L.) embryos to achieve high efficiency of oil biosynthesis.Journal of experimental botany · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pennycress (Thlaspi arvense L.), a plant naturalized to North America, accumulates high levels of erucic acid in its seeds, which makes it a promising biodiesel and industrial crop. The main carbon sinks in pennycress embryos were found to be proteins, fatty acids, and cell wall, which respectively represented 38.5, 33.2, and 27.0% of the biomass at 21 days after pollination. Erucic acid reached a maximum of 36% of the total fatty acids. Together these results indicate that total oil and erucic acid contents could be increased to boost the economic competitiveness of this crop. Understanding the biochemical basis of oil synthesis in pennycress embryos is therefore timely and relevant to guide future breeding and/or metabolic engineering efforts. For this purpose, a combination of metabolomics approaches was conducted to assess the active biochemical pathways during oil synthesis. First, gas chromatography-mass spectrometry (GC-MS) profiling of intracellular metabolites highlighted three main families of compounds: organic acids, amino acids, and sugars/sugar alcohols. Secondly, these intermediates were quantified in developing pennycress embryos by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in multiple reaction monitoring mode. Finally, partitional clustering analysis grouped the intracellular metabolites that shared a similar pattern of accumulation over time into eight clusters. This study underlined that: (i) sucrose might be stored rather than cleaved into hexoses; (ii) glucose and glutamine would be the main sources of carbon and nitrogen, respectively; and (iii) glycolysis, the oxidative pentose phosphate pathway, the tricarboxylic acid cycle, and the Calvin cycle were active in developing pennycress embryos.
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