ArticleJournal of experimental botany2015
Novel MtCEP1 peptides produced in vivo differentially regulate root development in Medicago truncatula.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 90 citations in OpenAlex.
- CEPR1 function across plant lineages: peptide hormone recognition by cereal crop orthologs and developmental roles in barley.Journal of experimental botany · 2025Article
- Exogenous Application of ENOD40 and CEP1 Peptides Boosts Symbiotic Signaling Gene Expression and Productivity in Common Bean.Plants (Basel, Switzerland) · 2025Article
- CEPR2 perceives group II CEPs to regulate cell surface receptor-mediated immunity in Arabidopsis.PLoS pathogens · 2025Article
- The white lupin CCR1 receptor-like kinase controls systemic Autoregulation of Cluster Root and Nodule Development.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Peptides and Reactive Oxygen Species Regulate Root Development.International journal of molecular sciences · 2025Review
- MicroRNA gatekeepers: Orchestrating rhizospheric dynamics.Journal of integrative plant biology · 2025Review
- Peptide hormones in plants.Molecular horticulture · 2025Review
- Bioinformatic and Phenotypic Analysis ofPlants (Basel, Switzerland) · 2024Article
- A Genome-Wide Analysis of the CEP Gene Family in Cotton and a Functional Study ofInternational journal of molecular sciences · 2024Article
- NPF and NRT2 fromPlants (Basel, Switzerland) · 2024Review
- CEP hormones at the nexus of nutrient acquisition and allocation, root development, and plant-microbe interactions.Journal of experimental botany · 2024Review
- Transmembrane proteins in grape immunity: current knowledge and methodological advances.Frontiers in plant science · 2024Review
- Bioassays for Identifying and Characterizing Plant Regulatory Peptides.Biomolecules · 2023Review
- Root-Knot-Nematode-Encoded CEPs Increase Nitrogen Assimilation.Life (Basel, Switzerland) · 2023Article
- The Medicago SymCEP7 hormone increases nodule number via shoots without compromising lateral root number.Plant physiology · 2023Article
- The small peptide CEP1 and the NIN-like protein NLP1 regulate NRT2.1 to mediate root nodule formation across nitrate concentrations.The Plant cell · 2023Article
- Identification and Expression Analysis of theInternational journal of molecular sciences · 2022Article
- Identification of Diverse Stress-Responsive Xylem Sap Peptides in Soybean.International journal of molecular sciences · 2022Article
- Progress in the Self-Regulation System in Legume Nodule Development-AON (Autoregulation of Nodulation).International journal of molecular sciences · 2022Review
- Metabolic Profiles Reveal Changes in the Leaves and Roots of Rapeseed (International journal of molecular sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small, post-translationally modified and secreted peptides regulate diverse plant developmental processes. Due to low natural abundance, it is difficult to isolate and identify these peptides. Using an improved peptide isolation protocol and Orbitrap mass spectrometry, nine 15-amino-acid CEP peptides were identified that corresponded to the two domains encoded by Medicago truncatula CEP1 (MtCEP1). Novel arabinosylated and hydroxylated peptides were identified in root cultures overexpressing MtCEP1. The five most abundant CEP peptides were hydroxylated and these species were detected also in low amounts in vector control samples. Synthetic peptides with different hydroxylation patterns differentially affected root development. Notably, the domain 1 peptide hydroxylated at Pro4 and Pro11 (D1:HyP4,11) imparted the strongest inhibition of lateral root emergence when grown with 5mM KNO3 and stimulated the highest increase in nodule number when grown with 0mM KNO3. Inhibition of lateral root emergence by D1:HyP4,11 was not alleviated by removing peptide exposure. In contrast, the domain 2 peptide hydroxylated at Pro11 (D2:HyP11) increased stage III-IV lateral root primordium numbers by 6-fold (P < 0.001) which failed to emerge. Auxin addition at levels which stimulated lateral root formation in wild-type plants had little or no ameliorating effect on CEP peptide-mediated inhibition of lateral root formation or emergence. Both peptides increased and altered the root staining pattern of the auxin-responsive reporter GH3:GUS suggesting CEPs alter auxin sensitivity or distribution. The results showed that CEP primary sequence and post-translational modifications influence peptide activities and the improved isolation procedure effectively and reproducibly identifies and characterises CEPs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.