ArticleTraffic (Copenhagen, Denmark)2015
Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae.
Article in Traffic (Copenhagen, Denmark), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 74 citations in OpenAlex.
- Article
- A minimal model for the role of Rim4 in regulating meiotic exit in budding yeast.Molecular biology of the cell · 2026Article
- Bni5 regulates and coordinates septin architecture and myosin-II functions at the cell division site.The Journal of cell biology · 2025Article
- The DNA replication checkpoint targets the kinetochore to reposition DNA structure-induced replication damage to the nuclear periphery.Cell reports · 2025Article
- Aneuploidy of specific chromosomes is beneficial to cells lacking spindle checkpoint protein Bub3.PLoS genetics · 2025Article
- Determination of Anaphase Duration by Time-Lapse Microscopy in Budding Yeast.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Spindle architecture constrains karyotype evolution.Nature cell biology · 2024Article
- Spindle architecture constrains karyotype in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- Reciprocal regulation by Elm1 and Gin4 controls septin hourglass assembly and remodeling.The Journal of cell biology · 2024Article
- A stable microtubule bundle formed through an orchestrated multistep process controls quiescence exit.eLife · 2024Article
- Unraveling the mechanisms and evolution of a two-domain module in IQGAP proteins for controlling eukaryotic cytokinesis.Cell reports · 2023Article
- Conserved roles for the dynein intermediate chain and Ndel1 in assembly and activation of dynein.Nature communications · 2023Article
- Microtubule-binding-induced allostery triggers LIS1 dissociation from dynein prior to cargo transport.Nature structural & molecular biology · 2023Article
- Meiosis II spindle disassembly requires two distinct pathways.Molecular biology of the cell · 2023Article
- Cik1 and Vik1 accessory proteins confer distinct functions to the kinesin-14 Kar3.Journal of cell science · 2023Article
- Conserved Roles for the Dynein Intermediate Chain and Ndel1 in Assembly and Activation of Dynein.bioRxiv : the preprint server for biology · 2023Article
- Using Budding Yeast as a Model to Understand Dynein-Mediated Cargo Transport.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exit.Biology open · 2022Article
- The microtubule plus-end tracking protein Bik1 is required for chromosome congression.Molecular biology of the cell · 2022Article
- Hierarchical integration of mitochondrial and nuclear positioning pathways by the Num1 EF hand.Molecular biology of the cell · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The ability to fluorescently label microtubules in live cells has enabled numerous studies of motile and mitotic processes. Such studies are particularly useful in budding yeast owing to the ease with which they can be genetically manipulated and imaged by live cell fluorescence microscopy. Because of problems associated with fusing genes encoding fluorescent proteins (FPs) to the native α-tubulin (TUB1) gene, the FP-Tub1 fusion is generally integrated into the genome such that the endogenous TUB1 locus is left intact. Although such modifications have no apparent consequences on cell viability, it is unknown if these genome-integrated FP-tubulin fusions negatively affect microtubule functions. Thus, a simple, economical and highly sensitive assay of microtubule function is required. Furthermore, the current plasmids available for generation of FP-Tub1 fusions have not kept pace with the development of improved FPs. Here, we have developed a simple and sensitive assay of microtubule function that is sufficient to identify microtubule defects that were not apparent by fluorescence microscopy or cell growth assays. Using results obtained from this assay, we have engineered a new family of 30 FP-Tub1 plasmids that use various improved FPs and numerous selectable markers that upon genome integration have no apparent defect on microtubule function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.