ArticlePloS one2015
The impairment of osteogenesis in bone sialoprotein (BSP) knockout calvaria cell cultures is cell density dependent.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Exploring fetal skeletal alterations induced by gold nanoparticles in mice confirmed by laser speckle imaging and LIBS approach.Biology direct · 2026Article
- Decoding the secretory blueprint of bone healing: from gradients to regeneration.Frontiers in cell and developmental biology · 2026Review
- OPN, BSP, and Bone Quality-Structural, Biochemical, and Biomechanical Assessment in OPNCalcified tissue international · 2024Article
- Functional defects in cementoblasts with disrupted bone sialoprotein functional domains, in vitro.Bone · 2024Article
- Osteogenic effects of rapamycin on bone marrow mesenchymal stem cells via inducing autophagy.Journal of orthopaedic surgery and research · 2023Article
- Fabrication of Interleukin-4 Encapsulated Bioactive Microdroplets for Regulating Inflammation and Promoting Osteogenesis.International journal of nanomedicine · 2023Article
- The role of Meteorin-like in skeletal development and bone fracture healing.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Osteogenic lithium-doped brushite cements for bone regeneration.Bioactive materials · 2022Article
- In Situ-Forming Collagen/poly-γ-glutamic Acid Hydrogel System with Mesenchymal Stem Cells and Bone Morphogenetic Protein-2 for Bone Tissue Regeneration in a Mouse Calvarial Bone Defect Model.Tissue engineering and regenerative medicine · 2022Article
- The Bone Sialoprotein RGD Domain Modulates and Maintains Periodontal Development.Journal of dental research · 2022Article
- FAM20C Overview: Classic and Novel Targets, Pathogenic Variants and Raine Syndrome Phenotypes.International journal of molecular sciences · 2021Review
- Effects ofMolecular medicine reports · 2021Article
- Evaluation of the Effects ofMedicina (Kaunas, Lithuania) · 2021Article
- Article
- AnStem cells international · 2021Review
- Influence of Maitake (BioMed research international · 2020Article
- Osteopontin is An Important Regulative Component of the Fetal Bone Marrow Hematopoietic Stem Cell Niche.Cells · 2019Article
- Mechanical strain triggers differentiation of dental mesenchymal stem cells by activating osteogenesis-specific biomarkers expression.American journal of translational research · 2019Article
- Transcriptome analysis of osteoblasts in an ovariectomized mouse model in response to physical exercise.Bone & joint research · 2018Article
- Expression and regulation of the ERK1/2 and p38 MAPK signaling pathways in periodontal tissue remodeling of orthodontic tooth movement.Molecular medicine reports · 2018Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone sialoprotein (BSP) belongs to the "small integrin-binding ligand N-linked glycoprotein" (SIBLING) family, whose members interact with bone cells and bone mineral. BSP is strongly expressed in bone and we previously showed that BSP knockout (BSP-/-) mice have a higher bone mass than wild type (BSP+/+) littermates, with lower bone remodelling. Because baseline bone formation activity is constitutively lower in BSP-/- mice, we studied the impact of the absence of BSP on in vitro osteogenesis in mouse calvaria cell (MCC) cultures. MCC BSP-/- cultures exhibit fewer fibroblast (CFU-F), preosteoblast (CFU-ALP) and osteoblast colonies (bone nodules) than wild type, indicative of a lower number of osteoprogenitors. No mineralized colonies were observed in BSP-/- cultures, along with little/no expression of either osteogenic markers or SIBLING proteins MEPE or DMP1. Osteopontin (OPN) is the only SIBLING expressed in standard density BSP-/- culture, at higher levels than in wild type in early culture times. At higher plating density, the effects of the absence of BSP were partly rescued, with resumed expression of osteoblast markers and cognate SIBLING proteins, and mineralization of the mutant cultures. OPN expression and amount are further increased in high density BSP-/- cultures, while PHEX and CatB expression are differentiatlly regulated in a manner that may favor mineralization. Altogether, we found that BSP regulates mouse calvaria osteoblast cell clonogenicity, differentiation and activity in vitro in a cell density dependent manner, consistent with the effective skeletogenesis but the low levels of bone formation observed in vivo. The BSP knockout bone microenvironment may alter the proliferation/cell fate of early osteoprogenitors.
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