ArticleApplied and environmental microbiology2015
Simple and portable magnetic immunoassay for rapid detection and sensitive quantification of plant viruses.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 64 citations in OpenAlex.
- Guided Electrokinetic Assembly of Functionalized Latex Beads for Fluorescence Signal Enhancement Using AC Electro-Osmosis.Sensors (Basel, Switzerland) · 2026Article
- Capacitive field-effect biosensors loaded with intact plant virus particles: modelling and experimental data.Analytical and bioanalytical chemistry · 2026Article
- Fundamentals and Applications of Dual-Frequency Magnetic Particle Spectroscopy: Review for Biomedicine and Materials Characterization.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Unleashing the power of colloidal gold immunochromatographic assays for plant virus diagnostics.MethodsX · 2024Review
- DNA Sensor for the Detection ofInternational journal of molecular sciences · 2023Article
- Magnetic Particle Spectroscopy for Point-of-Care: A Review on Recent Advances.Sensors (Basel, Switzerland) · 2023Review
- Five-Minute Magnetic Nanoparticle Spectroscopy-Based Bioassay for Ultrafast Detection of SARS-CoV-2 Spike Protein.ACS applied nano materials · 2022Article
- Review
- Frequency Mixing Magnetic Detection Setup Employing Permanent Ring Magnets as a Static Offset Field Source.Sensors (Basel, Switzerland) · 2022Article
- Magnetic nanoparticles and magnetic particle spectroscopy-based bioassays: a 15 year recap.Nano futures · 2022Article
- Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective.Plants (Basel, Switzerland) · 2022Review
- Nanozyme-based colorimetric biosensor with a systemic quantification algorithm for noninvasive glucose monitoring.Theranostics · 2022Review
- Role of different types of nanomaterials against diagnosis, prevention and therapy of COVID-19.Sustainable cities and society · 2021Review
- Detection of plant virus particles with a capacitive field-effect sensor.Analytical and bioanalytical chemistry · 2021Article
- Multiplex Detection of Magnetic Beads Using Offset Field Dependent Frequency Mixing Magnetic Detection.Sensors (Basel, Switzerland) · 2021Article
- Development of Fast and Portable Frequency Magnetic Mixing-Based Serological SARS-CoV-2-Specific Antibody Detection Assay.Frontiers in microbiology · 2021Article
- A Novel Method for Antibiotic Detection in Milk Based on Competitive Magnetic Immunodetection.Foods (Basel, Switzerland) · 2020Article
- Sensitive Aflatoxin B1 Detection Using Nanoparticle-Based Competitive Magnetic Immunodetection.Toxins · 2020Article
- Multiplex Detection of Different Magnetic Beads Using Frequency Scanning in Magnetic Frequency Mixing Technique.Sensors (Basel, Switzerland) · 2019Article
- 3D Printed Modular Immunofiltration Columns for Frequency Mixing-Based Multiplex Magnetic Immunodetection.Sensors (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant pathogens cause major economic losses in the agricultural industry because late detection delays the implementation of measures that can prevent their dissemination. Sensitive and robust procedures for the rapid detection of plant pathogens are therefore required to reduce yield losses and the use of expensive, environmentally damaging chemicals. Here we describe a simple and portable system for the rapid detection of viral pathogens in infected plants based on immunofiltration, subsequent magnetic detection, and the quantification of magnetically labeled virus particles. Grapevine fanleaf virus (GFLV) was chosen as a model pathogen. Monoclonal antibodies recognizing the GFLV capsid protein were immobilized onto immunofiltration columns, and the same antibodies were linked to magnetic nanoparticles. GFLV was quantified by immunofiltration with magnetic labeling in a double-antibody sandwich configuration. A magnetic frequency mixing technique, in which a two-frequency magnetic excitation field was used to induce a sum frequency signal in the resonant detection coil, corresponding to the virus concentration within the immunofiltration column, was used for high-sensitivity quantification. We were able to measure GFLV concentrations in the range of 6 ng/ml to 20 μg/ml in less than 30 min. The magnetic immunoassay could also be adapted to detect other plant viruses, including Potato virus X and Tobacco mosaic virus, with detection limits of 2 to 60 ng/ml.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.