ArticleBiochemistry2015
Substrate pathways in the nitrogenase MoFe protein by experimental identification of small molecule binding sites.
Article in Biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 62 citations in OpenAlex.
- Structural Characterization of Four Redox States of the P-cluster in Molybdenum Nitrogenase via Electrochemical Control of Crystals.Journal of the American Chemical Society · 2026Article
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- Nitrogen stable isotope fractionation by biological nitrogen fixation reveals cellular nitrogenase is diffusion limited.PNAS nexus · 2025Article
- Methylthio-alkane reductases use nitrogenase metalloclusters for carbon-sulfur bond cleavage.Nature catalysis · 2025Article
- Structural evolution of nitrogenase states under alkaline turnover.Nature communications · 2024Article
- CryoEM-sampling of metastable conformations appearing in cofactor-ligand association and catalysis of glutamate dehydrogenase.Scientific reports · 2024Article
- Mutational Analysis of the Nitrogenase Carbon Monoxide Protective Protein CowN Reveals That a Conserved C-Terminal Glutamic Acid Residue Is Necessary for Its Activity.Biochemistry · 2024Article
- Nitrogenase beyond the Resting State: A Structural Perspective.Molecules (Basel, Switzerland) · 2023Review
- Near ambient NNature reviews. Chemistry · 2023Review
- Structural consequences of turnover-induced homocitrate loss in nitrogenase.Nature communications · 2023Article
- Biological nitrogen fixation in theory, practice, and reality: a perspective on the molybdenum nitrogenase system.FEBS letters · 2023Review
- Structures of the nitrogenase complex prepared under catalytic turnover conditions.Science (New York, N.Y.) · 2022Article
- Statistical analysis of PRSC advances · 2022Article
- Review
- Small-Molecule Tunnels in Metalloenzymes Viewed as Extensions of the Active Site.Accounts of chemical research · 2021Review
- Structural Enzymology of Nitrogenase Enzymes.Chemical reviews · 2020Review
- Reactivity, Mechanism, and Assembly of the Alternative Nitrogenases.Chemical reviews · 2020Review
- Characterization of the Earliest Intermediate of Fe-NJournal of the American Chemical Society · 2019Article
- A Structural Basis forChemphyschem : a European journal of chemical physics and physical chemistry · 2019Article
- Mapping Hydrophobic Tunnels and Cavities in Neuroglobin with Noble Gas under Pressure.Biophysical journal · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In the nitrogenase molybdenum-iron (MoFe) protein, we have identified five potential substrate access pathways from the protein surface to the FeMo-cofactor (the active site) or the P-cluster using experimental structures of Xe pressurized into MoFe protein crystals from Azotobacter vinelandii and Clostridium pasteurianum. Additionally, all published structures of the MoFe protein, including those from Klebsiella pneumoniae, were analyzed for the presence of nonwater, small molecules bound to the protein interior. Each pathway is based on identification of plausible routes from buried small molecule binding sites to both the protein surface and a metallocluster. Of these five pathways, two have been previously suggested as substrate access pathways. While the small molecule binding sites are not conserved among the three species of MoFe protein, residues lining the pathways are generally conserved, indicating that the proposed pathways may be accessible in all three species. These observations imply that there is unlikely a unique pathway utilized for substrate access from the protein surface to the active site; however, there may be preferred pathways such as those described here.
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