ArticleJournal of biomedical optics2015
Intravital live cell triggered imaging system reveals monocyte patrolling and macrophage migration in atherosclerotic arteries.
Article in Journal of biomedical optics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Targeting monocytic Occludin impairs transendothelial migration and HIV neuroinvasion.EMBO reports · 2024Article
- Long COVID Definition, Symptoms, Risk Factors, Epidemiology and Autoimmunity: A Narrative Review.American journal of medicine open · 2024Article
- Cell Trafficking at the Intersection of the Tumor-Immune Compartments.Annual review of biomedical engineering · 2022Review
- Intravital Microscopy in Atherosclerosis Research.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nonclassical Monocytes (CD14dimCD16+) Are Associated With Carotid Intima-Media Thickness Progression for Men but Not Women: The Multi-Ethnic Study of Atherosclerosis-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- Macrophages: an indispensable piece of ovarian health.Biology of reproduction · 2021Review
- Trafficking of Mononuclear Phagocytes in Healthy Arteries and Atherosclerosis.Frontiers in immunology · 2021Review
- Hematopoiesis and Cardiovascular Disease.Circulation research · 2020Review
- Circulating BPIFB4 Levels Associate With and Influence the Abundance of Reparative Monocytes and Macrophages in Long Living Individuals.Frontiers in immunology · 2020Article
- Migratory and Dancing Macrophage Subsets in Atherosclerotic Lesions.Circulation research · 2019Article
- Myeloid cells in cardiovascular organs.Journal of internal medicine · 2019Review
- Limited Macrophage Positional Dynamics in Progressing or Regressing Murine Atherosclerotic Plaques-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Myeloid cell contributions to cardiovascular health and disease.Nature medicine · 2018Review
- Visualization of Monocytic Cells in Regressing Atherosclerotic Plaques by Intravital 2-Photon and Positron Emission Tomography-Based Imaging-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2018Article
- Resident and Monocyte-Derived Macrophages in Cardiovascular Disease.Circulation research · 2018Review
- Pre-metastatic cancer exosomes induce immune surveillance by patrolling monocytes at the metastatic niche.Nature communications · 2017Article
- Endothelial Protective Monocyte Patrolling in Large Arteries Intensified by Western Diet and Atherosclerosis.Circulation research · 2017Article
- A Polecat's View of Patrolling Monocytes.Circulation research · 2017Article
- Multiparametric Imaging of Organ System Interfaces.Circulation. Cardiovascular imaging · 2017Review
- MISTICA: Minimum Spanning Tree-Based Coarse Image Alignment for Microscopy Image Sequences.IEEE journal of biomedical and health informatics · 2016Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
Abstract
Intravital multiphoton imaging of arteries is technically challenging because the artery expands with every heartbeat, causing severe motion artifacts. To study leukocyte activity in atherosclerosis, we developed the intravital live cell triggered imaging system (ILTIS). This system implements cardiac triggered acquisition as well as frame selection and image registration algorithms to produce stable movies of myeloid cell movement in atherosclerotic arteries in live mice. To minimize tissue damage, no mechanical stabilization is used and the artery is allowed to expand freely. ILTIS performs multicolor high frame-rate two-dimensional imaging and full-thickness three-dimensional imaging of beating arteries in live mice. The external carotid artery and its branches (superior thyroid and ascending pharyngeal arteries) were developed as a surgically accessible and reliable model of atherosclerosis. We use ILTIS to demonstrate Cx3cr1GFP monocytes patrolling the lumen of atherosclerotic arteries. Additionally, we developed a new reporter mouse (Apoe−/−Cx3cr1GFP/+Cd11cYFP) to image GFP+ and GFP+YFP + macrophages “dancing on the spot” and YFP+ macrophages migrating within intimal plaque. ILTIS will be helpful to answer pertinent open questions in the field, including monocyte recruitment and transmigration, macrophage and dendritic cell activity, and motion of other immune cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.