ArticleInternational journal of ophthalmology2015
Early expression of mannose-binding lectin 2 during Aspergillus fumigatus infection in human corneal epithelial cells.
Article in International journal of ophthalmology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- ATF4 Involvement in TLR4 and LOX-1-Induced Host Inflammatory Response toJournal of ophthalmology · 2018Article
- Immune Recognition of Fungal Polysaccharides.Journal of fungi (Basel, Switzerland) · 2017Review
- Protein-carbohydrate interactions as part of plant defense and animal immunity.Molecules (Basel, Switzerland) · 2015Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the early expression of mannose-binding lectin 2 (MBL2) in human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus (AF).
methodsHCECs cultured in vitro with AF antigens and sampled at 0, 0.5, 1, 2, 4, 6 and 8h. The expression of MBL2 mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR). The expression of MBL2 protein in supernatant fluid was shown by enzyme linked immunosorbent assay (ELISA). MBL2 protein in HCECs was detected by immunocytochemistry at 0 and 24h.
resultsMBL2 mRNA and protein are expressed in normal HCECs. The expression of MBL2 mRNA and protein in supernatant fluid begin to increase after being stimulated with AF antigens. The most significantly peak of MBL2 mRNA is in 2h. The protein of MBL2 in supernatant fluid decrease gradually after 0.5h. The protein in HCECs expression increase after stimulation of 24h.
conclusionMBL2 receptor expressed in normal HCECs in vitro. The stimulation by AF antigens can increase the early expression of it.
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Registered trials
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