ArticleFrontiers in microbiology2015
Environmental monitoring of waterborne Campylobacter: evaluation of the Australian standard and a hybrid extraction-free MPN-PCR method.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- The Power of Old Hats: Rediscovering Inosine-EpPCR to Create Starting Libraries for Whole-Cell-SELEX.Biosensors · 2025Article
- Recent developments in waterborne pathogen detection technologies.Environmental monitoring and assessment · 2025Review
- FlashPCR: Revolutionising qPCR by Accelerating Amplification through Low ∆T Protocols.International journal of molecular sciences · 2024Article
- Article
- Universal Enzyme-Based Field Workflow for Rapid and Sensitive Quantification of Water Pathogens.Microorganisms · 2021Article
- Article
- Salmonella from a Microtidal Estuary Are Capable of Invading Human Intestinal Cell Lines.Microbial ecology · 2020Article
- Campylobacter in an Urban Estuary: Public Health Insights from Occurrence, HeLa Cytotoxicity, and Caco-2 Attachment Cum Invasion.Microbes and environments · 2019Article
- Review
- Article
- Evaluation of Techniques for Measuring Microbial Hazards in Bathing Waters: A Comparative Study.PloS one · 2016Article
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Authors and funding
10 authors.
Funding
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Abstract
Campylobacter is the leading agent of diarrheal disease worldwide. This study evaluates a novel culture-PCR hybrid (MPN-PCR) assay for the rapid enumeration of Campylobacter spp. from estuarine and wastewater systems. To first evaluate the current, culture-based, Australian standard, an inter-laboratory study was conducted on 69 subsampled water samples. The proposed Most-Probable Number (MPN)-PCR method was then evaluated, by analysing 147 estuarine samples collected over a 2 year period. Data for 14 different biological, hydrological and climatic parameters were also collated to identify pathogen-environment relationships and assess the potential for method specific bias. The results demonstrated that the intra-laboratory performance of the MPN-PCR was superior to that of AS/NZS (σ = 0.7912, P < 0.001; κ = 0.701, P < 0.001) with an overall diagnostic accuracy of ~94%. Furthermore, the analysis of both MPN-PCR and AS/NZS identified the potential for the introduction of method specific bias during assessment of the effects of environmental parameters on Campylobacter spp. numbers.
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Registered trials
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