ArticleFrontiers in microbiology2014
Interaction sites of DivIVA and RodA from Corynebacterium glutamicum.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Functional dissection ofmBio · 2026Article
- A dispensable SepIVA orthologue in Streptomyces venezuelae is associated with polar growth and not cell division.BMC microbiology · 2024Article
- MmpL3, Wag31, and PlrA are involved in coordinating polar growth with peptidoglycan metabolism and nutrient availability.Journal of bacteriology · 2024Article
- Article
- MmpL3, Wag31 and PlrA are involved in coordinating polar growth with peptidoglycan metabolism and nutrient availability.bioRxiv : the preprint server for biology · 2024Article
- Effects of benzothiazinone and ethambutol on the integrity of the corynebacterial cell envelope.Cell surface (Amsterdam, Netherlands) · 2023Article
- Eukaryotic-like gephyrin and cognate membrane receptor coordinate corynebacterial cell division and polar elongation.Nature microbiology · 2023Article
- Eukaryotic-like gephyrin and cognate membrane receptor coordinate corynebacterial cell division and polar elongation.bioRxiv : the preprint server for biology · 2023Article
- Article
- Localized Production of Cell Wall Precursors May Be Critical for Regulating the Mycobacterial Cell Wall.Journal of bacteriology · 2022Article
- Article
- Polar protein Wag31 both activates and inhibits cell wall metabolism at the poles and septum.Frontiers in microbiology · 2022Article
- Teichoic acids anchor distinct cell wall lamellae in an apically growing bacterium.Communications biology · 2020Article
- Article
- ¡vIVA la DivIVA!Journal of bacteriology · 2019Review
- Shedding Light on the Cell Biology of the Predatory BacteriumFrontiers in microbiology · 2019Article
- The transpeptidase PbpA and noncanonical transglycosylase RodA ofThe Journal of biological chemistry · 2018Article
- Detection of Protein Interactions in the Cytoplasm and Periplasm ofBio-protocol · 2018Article
- Genetic Dissection of DivIVA Functions in Listeria monocytogenes.Journal of bacteriology · 2017Article
- Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Elongation growth in actinobacteria is localized at the cell poles. This is in contrast to many classical model organisms where insertion of new cell wall material is localized around the lateral site. We previously described a role of RodA from Corynebacterium glutamicum in apical cell growth and morphogenesis. Deletion of rodA had drastic effects on morphology and growth, likely a result from misregulation of penicillin-binding proteins and cell wall precursor delivery. We identified the interaction of RodA with the polar scaffold protein DivIVA, thus explaining subcellular localization of RodA to the cell poles. In this study, we describe this interaction in detail and map the interaction sites of DivIVA and RodA. A single amino acid residue in the N-terminal domain of DivIVA was found to be crucial for the interaction with RodA. The interaction site of RodA was mapped to its cytoplasmic, C-terminal domain, in a region encompassing the last 10 amino acids (AAs). Deletion of these 10 AAs significantly decreased the interaction efficiency with DivIVA. Our results corroborate the interaction of DivIVA and RodA, underscoring the important role of DivIVA as a spatial organizer of the elongation machinery in Corynebacterineae.
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