ArticleCurrent microbiology2015
Diversity of human-associated Methanobrevibacter smithii isolates revealed by multispacer sequence typing.
Article in Current microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Tobacco-smoking-related prevalence of methanogens in the oral fluid microbiota.Scientific reports · 2018Trial
- Superior ability of dietary fiber utilization in obese breed pigs linked to gut microbial hydrogenotrophy.ISME communications · 2025Article
- Review
- Rapid identification of clinically interesting methanogens using an improved MALDI-TOF-MS assay.Access microbiology · 2022Article
- A Non-Invasive Neonatal Signature Predicts Later Development of Atopic Diseases.Journal of clinical medicine · 2022Article
- MeconialCurrent research in microbial sciences · 2021Article
- Diversity of Methanogens in Animals' Gut.Microorganisms · 2020Article
- Detection ofMicroorganisms · 2020Article
- Methanogens as emerging pathogens in anaerobic abscesses.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Review
- Co-culture of Methanobrevibacter smithii with enterobacteria during urinary infection.EBioMedicine · 2019Article
- Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity.Frontiers in public health · 2019Article
- Mutual Metabolic Interactions in Co-cultures of the IntestinalFrontiers in microbiology · 2019Article
- Methanobrevibacter smithii, a methanogen consistently colonising the newborn stomach.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Butyricimonas phoceensis sp. nov., a new anaerobic species isolated from the human gut microbiota of a French morbidly obese patient.New microbes and new infections · 2016Article
- Aerobic culture of methanogenic archaea without an external source of hydrogen.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016Article
- Noncontiguous finished genome sequence and description of Bacillus andreraoultii strain SIT1(T) sp. nov.New microbes and new infections · 2016Article
- Noncontiguous finished genome sequence and description of Paenibacillus ihumii sp. nov. strain AT5.New microbes and new infections · 2016Article
- Noncontiguous finished genome sequence and description of Kallipyga gabonensis sp. nov.New microbes and new infections · 2016Article
- Noncontiguous finished genome sequence and description of Gabonia massiliensis gen. nov., sp. nov.New microbes and new infections · 2016Article
- Rise of Microbial Culturomics: Noncontiguous Finished Genome Sequence and Description of Beduini massiliensis gen. nov., sp. nov.Omics : a journal of integrative biology · 2015Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methanobrevibacter smithii is the main archaea in human, detoxifying molecular hydrogen resulting from anaerobic bacteria fermentations into gaseous methane. Its identification relies on gene sequencing, but no method is available to discriminate among genetic variants of M. smithii. Here, we developed a multispacer sequence typing (MST) for genotyping the genetic variants of M. smithii. Four intergenic spacers recovered from the M. smithii reference genome were PCR amplified and sequenced in three M. smithii reference strains and in a collection of 22 M. smithii isolates from the oral cavity in two individuals and the gut of 10 additional individuals. Sequencing yielded 216 genetic polymorphisms including 89 single nucleotide polymorphisms (41.2 %), 83 insertions (38.4 %), and 44 deletions (20.4 %). Combining these genetic polymorphisms yielded 15 genotypes with an index of discrimination of 0.942 (confidence interval 0.9-0.984; P < 0.05). Five M. smithii isolates made from the oral cavity yielded five different genotypes; seven gut isolates yielded nine different genotypes; genotypes MST5 and MST6 were found both in the oral cavity and the gut. Multiple genotypes were identified in some individuals at the same anatomical site. MST is a sequencing-based method which discriminates several genetic variants within M. smithii. Individuals may harbor several contemporary genetic variants of M. smithii in the oral cavity and gut. MST will allow studying population dynamics of M. smithii and tracing its circulation between individuals and their environment.
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