ArticleScientific reports2015
Design and application of a novel high-throughput screening technique for 1-deoxynojirimycin.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 37 citations in OpenAlex.
- In Vivo Functional Investigation of LysC3 (Aspartokinase 3) Revealed Its Role in Priming the Biosynthesis of 1-Deoxynojirimycin in Mulberry.Plants (Basel, Switzerland) · 2026Article
- Soil Application ofMetabolites · 2024Article
- Protein engineering of transaminase facilitating enzyme cascade reaction for the biosynthesis of azasugars.iScience · 2024Article
- Engineering and application of LacI mutants with stringent expressions.Microbial biotechnology · 2024Article
- Biocatalytic Cascades toward Iminosugar Scaffolds Reveal Promiscuous Activity of Shikimate Dehydrogenases.ACS central science · 2023Article
- Valorisation of By-Products from Soybean (Molecules (Basel, Switzerland) · 2020Review
- Improved production of 1-deoxynojirymicin in Escherichia coli through metabolic engineering.World journal of microbiology & biotechnology · 2018Article
- An Oil-Free Picodrop Bioassay Platform for Synthetic Biology.Scientific reports · 2018Article
- Design and application of a lactulose biosensor.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput screening techniques for small molecules can find intensive applications in the studies of biosynthesis of these molecules. A sensitive, rapid and cost-effective technique that allows high-throughput screening of endogenous production of the natural iminosugar 1-deoxynojirimycin (1-DNJ), an α-glucosidase inhibitor relevant to the pharmaceutical industry, was developed in this study, based on the inhibitory effects of 1-DNJ on the activity of the β-glycosidase LacS from Sulfolobus solfataricus. This technique has been demonstrated effective in engineering both the key enzyme and the expression levels of enzymes in the 1-DNJ biosynthetic pathway from Bacillus atrophaeus cloned in E. coli. Higher biosynthetic efficiency was achieved using directed evolution strategies.
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