ArticleMolecular and cellular biochemistry2015
Simultaneous oral and inhalational intake of molecular hydrogen additively suppresses signaling pathways in rodents.
Article in Molecular and cellular biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 51 citations in OpenAlex.
- Molecular Hydrogen Attenuates Chronic Inflammation and Delays the Onset of Ultraviolet B-Induced Skin Carcinogenesis in Mice.International journal of molecular sciences · 2026Article
- The Rieske iron-sulfur protein is a primary target of molecular hydrogen.Redox biology · 2025Article
- Molecular mechanisms associated with effects of hydrogen molecule in liver diseases: the review of current evidence.European journal of medical research · 2025Review
- Hydrogen-rich electrolyzed water is a useful mouthwash due to its biofilm-control properties: an in vitro and in vivo study.Journal of Yeungnam medical science · 2025Article
- Oral Administration of Hydrogen-rich Water: Biomedical Activities, Potential Mechanisms, and Clinical Applications.Current pharmaceutical design · 2025Review
- Oral Intake of Hydrogen Water Improves Retinal Blood Flow Dysregulation in Response to Flicker Stimulation and Systemic Hyperoxia in Diabetic Mice.Translational vision science & technology · 2024Article
- Molecular Hydrogen: From Molecular Effects to Stem Cells Management and Tissue Regeneration.Antioxidants (Basel, Switzerland) · 2023Review
- Effects of hydrogen-rich saline in neuroinflammation and mitochondrial dysfunction in rat model of sepsis-associated encephalopathy.Journal of translational medicine · 2022Article
- Electrolyzed-Reduced Water: Review I. Molecular Hydrogen Is the Exclusive Agent Responsible for the Therapeutic Effects.International journal of molecular sciences · 2022Review
- Hydrogen-Rich Water Mitigates LPS-Induced Chronic Intestinal Inflammatory Response in Rats via Nrf-2 and NF-κB Signaling Pathways.Veterinary sciences · 2022Article
- AccurateHeliyon · 2022Article
- Antioxidation activity of molecular hydrogen via protoheme catalysis in vivo: an insight from ab initio calculations.Journal of molecular modeling · 2022Article
- Hydrogen, a Novel Therapeutic Molecule, Regulates Oxidative Stress, Inflammation, and Apoptosis.Frontiers in physiology · 2021Review
- Molecular and Cellular Mechanisms Associated with Effects of Molecular Hydrogen in Cardiovascular and Central Nervous Systems.Antioxidants (Basel, Switzerland) · 2020Review
- Hydrogen: An Endogenous Regulator of Liver Homeostasis.Frontiers in pharmacology · 2020Review
- Preventive Effects of Drinking Hydrogen-Rich Water on Gingival Oxidative Stress and Alveolar Bone Resorption in Rats Fed a High-Fat Diet.Nutrients · 2017Article
- Molecular Hydrogen as a Neuroprotective Agent.Current neuropharmacology · 2017Review
- Molecular hydrogen suppresses activated Wnt/β-catenin signaling.Scientific reports · 2016Article
- Effects of hydrogen-rich saline on early acute kidney injury in severely burned rats by suppressing oxidative stress induced apoptosis and inflammation.Journal of translational medicine · 2015Article
- Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive review of 321 original articles.Medical gas research · 2015Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular hydrogen (H2) is an agent with potential applications in oxidative stress-related and/or inflammatory disorders. H2 is usually administered by inhaling H2-containing air (HCA) or by oral intake of H2-rich water (HRW). Despite mounting evidence, the molecular mechanism underlying the therapeutic effects and the optimal method of H2 administration remain unclear. Here, we investigated whether H2 affects signaling pathways and gene expression in a dosage- or dose regimen-dependent manner. We first examined the H2 concentrations in blood and organs after its administration and found that oral intake of HRW rapidly but transiently increased H2 concentrations in the liver and atrial blood, while H2 concentrations in arterial blood and the kidney were one-tenth of those in the liver and atrial blood. In contrast, inhalation of HCA increased H2 equally in both atrial and arterial blood. We next examined whether H2 alters gene expression in normal mouse livers using DNA microarray analysis after administration of HCA and HRW. Ingenuity Pathway Analysis revealed that H2 suppressed the expression of nuclear factor-kappa B (NF-κB)-regulated genes. Western blot analysis showed that H2 attenuated ERK, p38 MAPK, and NF-κB signaling in mouse livers. Finally, we evaluated whether the changes in gene expression were influenced by the route of H2 administration and found that the combination of both HRW and HCA had the most potent effects on signaling pathways and gene expression in systemic organs, suggesting that H2 may act not only through a dose-dependent mechanism but also through a complex molecular network.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.