ArticleArchives of biochemistry and biophysics2015
Gadolinium modifies the cell membrane to inhibit permeabilization by nanosecond electric pulses.
Article in Archives of biochemistry and biophysics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection.EMBO molecular medicine · 2024Article
- Real-time imaging of individual electropores proves their longevity in cells.Biochemical and biophysical research communications · 2024Article
- Dynamics of cell membrane lesions and adaptive conductance under the electrical stress.Cell stress · 2024Article
- Next generation CANCAN focusing for remote stimulation by nanosecond electric pulses.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Rapid and precise tracking of water influx and efflux across cell membranes induced by a pulsed electric field.Biomedical optics express · 2023Article
- Propidium uptake and ATP release in A549 cells share similar transport mechanisms.Biophysical journal · 2022Article
- Electroporation and cell killing by milli- to nanosecond pulses and avoiding neuromuscular stimulation in cancer ablation.Scientific reports · 2022Article
- Benefits and Detriments of Gadolinium from Medical Advances to Health and Ecological Risks.Molecules (Basel, Switzerland) · 2020Review
- Nanosecond pulsed electric fields modulate the expression of the astaxanthin biosynthesis genes psy, crtR-b and bkt 1 in Haematococcus pluvialis.Scientific reports · 2020Article
- Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of CaInternational journal of molecular sciences · 2020Article
- Orally administered gadolinium orthovanadate GdVOWiener medizinische Wochenschrift (1946) · 2020Article
- Cancellation of nerve excitation by the reversal of nanosecond stimulus polarity and its relevance to the gating time of sodium channels.Cellular and molecular life sciences : CMLS · 2019Article
- The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency.Bioelectrochemistry (Amsterdam, Netherlands) · 2018Article
- Electropermeabilization of cells by closely spaced paired nanosecond-range pulses.Bioelectrochemistry (Amsterdam, Netherlands) · 2018Article
- Delayed hypersensitivity to nanosecond pulsed electric field in electroporated cells.Scientific reports · 2017Article
- Neuronal excitation and permeabilization by 200-ns pulsed electric field: An optical membrane potential study with FluoVolt dye.Biochimica et biophysica acta. Biomembranes · 2017Article
- Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.Cellular and molecular life sciences : CMLS · 2017Article
- Electroporation by subnanosecond pulses.Biochemistry and biophysics reports · 2016Article
- Electrosensitization assists cell ablation by nanosecond pulsed electric field in 3D cultures.Scientific reports · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Lanthanide ions are the only known blockers of permeabilization by electric pulses of nanosecond duration (nsEP), but the underlying mechanisms are unknown. We employed timed applications of Gd(3+) before or after nsEP (600-ns, 20 kV/cm) to investigate the mechanism of inhibition, and measured the uptake of the membrane-impermeable YO-PRO-1 (YP) and propidium (Pr) dyes. Gd(3+) inhibited dye uptake in a concentration-dependent manner. The inhibition of Pr uptake was always about 2-fold stronger. Gd(3+) was effective when added after nsEP, as well as when it was present during nsEP exposure and removed afterward. Pores formed by nsEP in the presence of Gd(3+) remained quiescent unless Gd(3+) was promptly washed away. Such pores resealed (or shrunk) shortly after the wash despite the absence of Gd(3+). Finally, a brief (3s) Gd(3+) perfusion was equally potent at inhibiting dye uptake when performed either immediately before or after nsEP, or early before nsEP. The persistent protective effect of Gd(3+) even in its absence proves that inhibition by Gd(3+) does not result from simple pore obstruction. Instead, Gd(3+) causes lasting modification of the membrane, occurring promptly and irrespective of pore presence; it makes the membrane less prone to permeabilization and/or reduces the stability of electropores.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.