ArticlePloS one2015
A transcriptome derived female-specific marker from the invasive Western mosquitofish (Gambusia affinis).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Production of YY super-males in Pseudobagrus ussuriensis using estradiol-induced XY pseudo-females.Fish physiology and biochemistry · 2026Article
- Article
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- Sex determination mechanisms and sex control approaches in aquaculture animals.Science China. Life sciences · 2022Review
- Chromosome-level genome assembly of the female western mosquitofish (Gambusia affinis).GigaScience · 2020Article
- Transcriptomic changes in western mosquitofish (Gambusia affinis) liver following benzo[a]pyrene exposure.Environmental science and pollution research international · 2020Article
- Independent Origin of XY and ZW Sex Determination Mechanisms in Mosquitofish Sister Species.Genetics · 2020Article
- Sex-specific markers developed by next-generation sequencing confirmed an XX/XY sex determination system in bighead carp (Hypophthalmichehys nobilis) and silver carp (Hypophthalmichthys molitrix).DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- The gonadal transcriptome of the unisexual Amazon molly Poecilia formosa in comparison to its sexual ancestors, Poecilia mexicana and Poecilia latipinna.BMC genomics · 2018Article
- Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3´ UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sex-specific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.
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