ArticlePloS one2015
Differential expression of metallothionein isoforms in terrestrial snail embryos reflects early life stage adaptation to metal stress.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 35 citations in OpenAlex.
- Metals and metallothionein evolution in snails: a contribution to the concept of metal-specific functionality from an animal model group.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Review
- Effects of Cadmium Sulfate on the Brown Garden SnailToxics · 2021Article
- Seasonal variation of biomarker responses in Cantareus aspersus and physic-chemical properties of soils from Northeast Algeria.Environmental science and pollution research international · 2020Article
- Biological and biochemical assessment in Phorcus articulatus (Lamarck 1822): contamination and seasonal effect.Environmental monitoring and assessment · 2019Article
- Unravelling the Role of Metallothionein on Development, Reproduction and Detoxification in the Wall Lizard Podarcis sicula.International journal of molecular sciences · 2017Review
- Effect of cadmium on cytosine hydroxymethylation in gastropod hepatopancreas.Environmental science and pollution research international · 2017Article
- High-level dietary cadmium exposure is associated with global DNA hypermethylation in the gastropod hepatopancreas.PloS one · 2017Article
- Genetic Diversity in Insect Metal Tolerance.Frontiers in genetics · 2017Review
- Cadmium Accumulation and Pathological Alterations in the Midgut Gland of Terrestrial Snail Helix pomatia L. from a Zinc Smelter Area: Role of Soil pH.Bulletin of environmental contamination and toxicology · 2016Article
- Differential sensitivity of snail embryos to cadmium: relation to age and metallothionein gene expression.Environmental science and pollution research international · 2016Article
- Metallothionein gene expression in embryos of the terrestrial snail (Cantareus aspersus) exposed to cadmium and copper in the Bordeaux mixture.Environmental science and pollution research international · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The aim of this study was to analyze the expression of three metallothionein (MT) isoform genes (CdMT, CuMT and Cd/CuMT), already known from adults, in the Early Life Stage (ELS) of Cantareus aspersus. This was accomplished by detection of the MT isoform-specific transcription adopting Polymerase Chain Reaction (PCR) amplification and quantitative Real Time (qRT)-PCR of the three MT genes. Freshly laid eggs were kept for 24 hours under control conditions or exposed to three cadmium (Cd) solutions of increasing concentration (5, 10, and 15 mg Cd/L). The transcription of the three MT isoform genes was detected via PCR in 1, 6 and 12-day-old control or Cd-exposed embryos. Moreover, the transcription of this isoform genes during development was followed by qRT-PCR in 6 and 12-day-old embryos. Our results showed that the CdMT and Cd/CuMT genes, but not the CuMT gene, are expressed in embryos at the first day of development. The transcription of the 3 MT genes in control embryos increased with development time, suggesting that the capacities of metal regulation and detoxification may have gradually increased throughout embryogenesis. However in control embryos, the most highly expressed MT gene was that of the Cd/CuMT isoform, whose transcription levels greatly exceeded those of the other two MT genes. This contrasts with the minor significance of this gene in adult snails and suggests that in embryos, this isoform may play a comparatively more important role in metal physiology compared to adult individuals. This function in adult snails appears not to be related to Cd detoxification. Instead, snail embryos responded to Cd exposure by over-expression of the CdMT gene in a concentration-dependent manner, whereas the expression of the Cd/CuMT gene remained unaffected. Moreover, our study demonstrates the ability of snail embryos to respond very early to Cd exposure by up-regulation of the CdMT gene.
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