ArticlePloS one2015
Proteomic analysis of pure human airway gland mucus reveals a large component of protective proteins.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 54 citations in OpenAlex.
- Distinct Functional Signatures of Human Olfactory and Respiratory Mucus Revealed by Proteomics Combined With Machine Learning.Proteomics. Clinical applications · 2026Article
- Structural and cellular mechanisms of mucus plugging in the larger airways.Chinese medical journal pulmonary and critical care medicine · 2026Review
- Proteomic profiling of equine airway mucus reveals compositional changes in asthmatic phenotypes.Scientific reports · 2026Article
- Porcine airway tissue explant culture at a hydrogel-air interface to model submucosal glands in cystic fibrosis progression and therapy.Science advances · 2025Article
- Galectin-3: a novel antimicrobial host factor identified in goat nasal mucus.Veterinary research · 2025Article
- Evaluation of the decontamination efficacy of a portable air cleaner using 275-nm UVC-LED radiation against airborne Coronavirus and Influenza virus.GMS hygiene and infection control · 2025Article
- Dynamic measurement of airway surface liquid volume with anLab on a chip · 2024Article
- Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations.European journal of microbiology & immunology · 2024Review
- Mucus Structure, Viscoelastic Properties, and Composition in Chronic Respiratory Diseases.International journal of molecular sciences · 2024Review
- Stability of Feline Coronavirus in aerosols and dried in organic matrices on surfaces at various environmental conditions.Scientific reports · 2023Article
- Proteome of airway surface liquid and mucus in newborn wildtype and cystic fibrosis piglets.Respiratory research · 2023Article
- Article
- Physiology and pathophysiology of human airway mucus.Physiological reviews · 2022Review
- Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.Science advances · 2022Article
- Cellular and molecular architecture of submucosal glands in wild-type and cystic fibrosis pigs.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Frontline workers: Mediators of mucosal immunity in community acquired pneumonia and COVID-19.Frontiers in immunology · 2022Review
- Respiratory mucus as a virus-host range determinant.Trends in microbiology · 2021Review
- Combined agonists act synergistically to increase mucociliary clearance in a cystic fibrosis airway model.Scientific reports · 2021Article
- Unveiling Putative Functions of Mucus Proteins and Their Tryptic Peptides in Seven Gastropod Species Using Comparative Proteomics and Machine Learning-Based Bioinformatics Predictions.Molecules (Basel, Switzerland) · 2021Article
- PAR-2-activated secretion by airway gland serous cells: role for CFTR and inhibition byAmerican journal of physiology. Lung cellular and molecular physiology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Airway submucosal glands contribute to innate immunity and protect the lungs by secreting mucus, which is required for mucociliary clearance and which also contains antimicrobial, anti-inflammatory, anti-proteolytic and anti-oxidant proteins. We stimulated glands in tracheal trimmings from three lung donors and collected droplets of uncontaminated mucus as they formed at the gland orifices under an oil layer. We analyzed the mucus using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analysis identified 5486 peptides and 441 proteins from across the 3 samples (269-319 proteins per subject). We focused on 269 proteins common to at least 2 0f 3 subjects, of which 102 (38%) had protective or innate immunity functions. While many of these have long been known to play such roles, for many others their cellular protective functions have only recently been appreciated in addition to their well-studied biologic functions (e.g. annexins, apolipoproteins, gelsolin, hemoglobin, histones, keratins, and lumican). A minority of the identified proteins are known to be secreted via conventional exocytosis, suggesting that glandular secretion occurs via multiple mechanisms. Two of the observed protective proteins, major vault protein and prohibitin, have not been observed in fluid from human epithelial cultures or in fluid from nasal or bronchoalveolar lavage. Further proteomic analysis of pure gland mucus may help clarify how healthy airways maintain a sterile environment.
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Registered trials
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