ArticlePLoS neglected tropical diseases2015
Comparison of two quantitative real time PCR assays for Rickettsia detection in patients from Tunisia.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 39 citations in OpenAlex.
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- Aetiologies of bacterial tick-borne febrile illnesses in humans in Africa: diagnostic limitations and the need for improvement.Frontiers in medicine · 2024Review
- Human-biting ticks and zoonotic tick-borne pathogens in North Africa: diversity, distribution, and trans-Mediterranean public health challenges.One health (Amsterdam, Netherlands) · 2023Review
- Genetic typing of isolates of Rickettsia typhi.PLoS neglected tropical diseases · 2022Article
- Molecular Evidence for Flea-Borne Rickettsiosis in Febrile Patients from Madagascar.Pathogens (Basel, Switzerland) · 2021Article
- An Update on the Laboratory Diagnosis ofPathogens (Basel, Switzerland) · 2021Review
- Empirical treatment with doxycycline of fever of intermediate duration.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Spotted fever diagnosis: Experience from a South Indian center.Pathogens and global health · 2021Article
- Epidemiology, Clinical Aspects, Laboratory Diagnosis and Treatment of Rickettsial Diseases in the Mediterranean Area During COVID-19 Pandemic: A Review of the Literature.Mediterranean journal of hematology and infectious diseases · 2020Review
- Diagnosis of spotted fever groupEpidemiology and infection · 2019Review
- Evidence of rickettsiae in Danish patients tested for Lyme neuroborreliosis: a retrospective study of archival samples.BMC infectious diseases · 2018Article
- State of the art of diagnosis of rickettsial diseases: the use of blood specimens for diagnosis of scrub typhus, spotted fever group rickettsiosis, and murine typhus.Current opinion in infectious diseases · 2016Review
- Acute febrile illness is associated with Rickettsia spp infection in dogs.Parasites & vectors · 2015Article
- Probable scrub typhus-like infection in Colombia: seroconversion and serological reactivity to Orientia spp. among patients with acute undifferentiated febrile illness in Villeta municipality.Travel medicine and infectious diseaseArticle
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectivesQuantitative real time PCR (qPCR) offers rapid diagnosis of rickettsial infections. Thus, successful treatment could be initiated to avoid unfavorable outcome. Our aim was to compare two qPCR assays for Rickettsia detection and to evaluate their contribution in early diagnosis of rickettsial infection in Tunisian patients. PATIENTS AND
methodsIncluded patients were hospitalized in different hospitals in Tunisia from 2007 to 2012. Serology was performed by microimmunofluorescence assay using R. conorii and R. typhi antigens. Two duplex qPCRs, previously reported, were performed on collected skin biopsies and whole blood samples. The first duplex amplified all Rickettsia species (PanRick) and Rickettsia typhi DNA (Rtt). The second duplex detected spotted fever group Rickettsiae (RC00338) and typhus group Rickettsiae DNA (Rp278).
resultsDiagnosis of rickettsiosis was confirmed in 82 cases (57.7%). Among 44 skin biopsies obtained from patients with confirmed diagnosis, the first duplex was positive in 24 samples (54.5%), with three patients positive by Rtt qPCR. Using the second duplex, positivity was noted in 21 samples (47.7%), with two patients positive by Rp278 qPCR. Among79 whole blood samples obtained from patients with confirmed diagnosis, panRick qPCR was positive in 5 cases (6.3%) among which two were positive by Rtt qPCR. Using the second set of qPCRs, positivity was noted in four cases (5%) with one sample positive by Rp278 qPCR. Positivity rates of the two duplex qPCRs were significantly higher among patients presenting with negative first serum than those with already detectable antibodies.
conclusionsUsing qPCR offers a rapid diagnosis. The PanRick qPCR showed a higher sensitivity. Our study showed that this qPCR could offer a prompt diagnosis at the early stage of the disease. However, its implementation in routine needs cost/effectiveness evaluation.
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