Evidence map›Paper›PMID 25705067›Full record

ArticleModern physics letters. B, Condensed matter physics, statistical physics, applied physics2014

Understanding dynamic changes in live cell adhesion with neutron reflectometry.

Ann Junghans, Mary Jo Waltman, Hillary L Smith, Luka Pocivavsek, Noureddine Zebda, Konstantin Birukov, Mariano Viapiano, Jaroslaw Majewski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Modern physics letters. B, Condensed matter physics, statistical physics, applied physics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ann JunghansMPA-CINT/Lujan Neutron Scattering Center, Los Alamos Neutron Science Center, Los Alamos National Laboratory, Los Alamos, New Mexico, 87545, USA.
Mary Jo WaltmanBiosciences Division, Bioenergy and Biome Sciences, Los Alamos National Laboratory, Los Alamos, New Mexico, 87545, USA.
Hillary L SmithDepartment of Applied Physics and Materials Science, California Institute of Technology, Pasadena, CA 91125, USA.
Luka PocivavsekDepartment of Surgery, University of Pittsburgh Medical Center, 200 Lothrop St, Pittsburgh, PA 15213, USA.
Noureddine ZebdaNDA Analytics, Woolley Road, Huntingdon, Cambridgeshire, PE28 4HS, UK.
Konstantin BirukovLung Injury Center, Department of Medicine, The University of Chicag; 5841 S. Maryland Ave., Chicago, IL 60637, USA.
Mariano ViapianoDepartment of Neurosurgery, Brigham and Women's Hospital and Harvard Medical School 4 Blackfan Circle, Boston, MA 02115, USA.
Jaroslaw MajewskiMPA-CINT/Lujan Neutron Scattering Center, Los Alamos Neutron Science Center, Los Alamos National Laboratory, Los Alamos, New Mexico, 87545, USA.

Funding

Control of Lung Permeability by Oxidized PhospholipidsR01HL076259 · NHLBI · UNIVERSITY OF MARYLAND BALTIMORE · PI BIRUKOV, KONSTANTIN · 2004 to 2023
$6.8M
Targeting the Tumor Matrix as Anti-Invasive and Sensitizing Strategy for GliomaR01CA152065 · NCI · OHIO STATE UNIVERSITY · PI VIAPIANO, MARIANO SEBASTIAN · 2010 to 2014
$1.3M
NCI NIH HHS R01 CA152065NHLBI NIH HHS R01 HL076259
6 · The paper itself

Abstract

Neutron reflectometry (NR) was used to examine various live cells adhesion to quartz substrates under different environmental conditions, including flow stress. To the best of our knowledge, these measurements represent the first successful visualization and quantization of the interface between live cells and a substrate with sub-nanometer resolution. In our first experiments, we examined live mouse fibroblast cells as opposed to past experiments using supported lipids, proteins, or peptide layers with no associated cells. We continued the NR studies of cell adhesion by investigating endothelial monolayers and glioblastoma cells under dynamic flow conditions. We demonstrated that neutron reflectometry is a powerful tool to study the strength of cellular layer adhesion in living tissues, which is a key factor in understanding the physiology of cell interactions and conditions leading to abnormal or disease circumstances. Continuative measurements, such as investigating changes in tumor cell - surface contact of various glioblastomas, could impact advancements in tumor treatments. In principle, this can help us to identify changes that correlate with tumor invasiveness. Pursuit of these studies can have significant medical impact on the understanding of complex biological problems and their effective treatment,

Indexed as

adhesioncellsendothelial monolayerglioblastomasneutron scatteringshear stress

Identifiers

PMID25705067
PMCPMC4334466

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.