ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2015
Evaluating the efficiency of isotope transmission for improved panel design and a comparison of the detection sensitivities of mass cytometer instruments.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 70 citations in OpenAlex.
- Quantitative single cell analysis of gold nanoparticle association across a mouse triple-negative breast cancer cell line and blood immune cell populations.Analytical and bioanalytical chemistry · 2026Article
- Reducing Bias in Mass Cytometry Data through Corrections for Spillover and Nonspecific Binding.Analytical chemistry · 2026Article
- High-throughput multiplexed serology via the mass-spectrometric analysis of isotopically barcoded beads.Nature biomedical engineering · 2025Article
- Cynomolgus macaques as a translational model of human immune responses to yellow fever 17D vaccination.Journal of virology · 2024Article
- Approaching Mass Cytometry Translational Studies by Experimental and Data Curation Settings.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Expanded vacuum-stable gels for multiplexed high-resolution spatial histopathology.Nature communications · 2023Article
- Full spectrum flow cytometry and mass cytometry: A 32-marker panel comparison.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2022Article
- CyTOFFrontiers in immunology · 2022Review
- Immune Profiling Mass Cytometry Assay Harmonization: Multicenter Experience from CIMAC-CIDC.Clinical cancer research : an official journal of the American Association for Cancer Research · 2021Article
- Nonspecific Binding Correction for Single-Cell Mass Cytometric Analysis of Autophagy and Myoblast Differentiation.Analytical chemistry · 2021Article
- Vaccine Inoculation Route Modulates Early Immunity and Consequently Antigen-Specific Immune Response.Frontiers in immunology · 2021Article
- Article
- Method for the Analysis of the Tumor Microenvironment by Mass Cytometry: Application to Chronic Lymphocytic Leukemia.Frontiers in immunology · 2020Article
- Challenges in the Multivariate Analysis of Mass Cytometry Data: The Effect of Randomization.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).European journal of immunology · 2019Article
- Titrating Complex Mass Cytometry Panels.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Beyond the message: advantages of snapshot proteomics with single-cell mass cytometry in solid tumors.The FEBS journal · 2019Review
- Optimization of Receptor Occupancy Assays in Mass Cytometry: Standardization Across Channels with QSC Beads.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
- Methods for analyzing tellurium imaging mass cytometry data.PloS one · 2019Article
- Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry.Nature protocols · 2018Article
Corrections and comments
- Commented on by
Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent introduction of mass cytometry, a technique coupling a cell introduction system generating a stream of single cells with mass spectrometry, has greatly increased the number of parameters that can be measured per single cell. As with all new technology there is a need for dissemination of standardization and quality control procedures. Here, we characterize variations in sensitivity observed across the mass range of a mass cytometer, using different lanthanide tags. We observed a five-fold difference in lanthanide detection over the mass range and demonstrated that each instrument has its own sensitivity pattern. Therefore, the selection of lanthanide combinations is a key step in the establishment of a staining panel for mass cytometry-based experiments, particularly for multicenter studies. We propose the sensitivity pattern as the basis for panel design, instrument standardization and future implementation of normalization algorithms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.