Observational studyJournal of virological methods2015
A generic assay for whole-genome amplification and deep sequencing of enterovirus A71.
Observational study in Journal of virological methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Retrospective Genotyping of Enteroviruses Using a Diagnostic Nanopore Sequencing Workflow.Pathogens (Basel, Switzerland) · 2024Article
- Laboratory diagnosis of nonpolio enteroviruses: A review of the current literature.Biosafety and health · 2023Review
- Development of a Universal Cloning System for Reverse Genetics of Human Enteroviruses.Microbiology spectrum · 2023Article
- Clinical, etiological and epidemiological investigations of hand, foot and mouth disease in southern Vietnam during 2015 - 2018.PLoS neglected tropical diseases · 2020Article
- Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing.Viruses · 2020Article
- Seroprevalence of EV-A71 neutralizing antibodies following the 2011 epidemic in HCMC, Vietnam.PLoS neglected tropical diseases · 2020Article
- Neutralizing Antibodies against Enteroviruses in Patients with Hand, Foot and Mouth Disease.Emerging infectious diseases · 2020Article
- Incidence of Infection of Enterovirus 71 and Coxsackieviruses A6 and A16 among Household Contacts of Index Cases in Dong Thap Province, Southern Vietnam.BioMed research international · 2020Article
- Transmission and serotype features of hand foot mouth disease in household contacts in Dong Thap, Vietnam.BMC infectious diseases · 2019Article
- Rapid and Cost-Efficient Enterovirus Genotyping from Clinical Samples Using Flongle Flow Cells.Genes · 2019Article
- Economic Burden Attributed to Children Presenting to Hospitals With Hand, Foot, and Mouth Disease in Vietnam.Open forum infectious diseases · 2019Article
- Enterovirus A71 Phenotypes Causing Hand, Foot and Mouth Disease, Vietnam.Emerging infectious diseases · 2019Article
- Severe enterovirus A71 associated hand, foot and mouth disease, Vietnam, 2018: preliminary report of an impending outbreak.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2018Article
- Whole Genome Sequencing of Enteroviruses Species A to D by High-Throughput Sequencing: Application for Viral Mixtures.Frontiers in microbiology · 2018Article
- Molecular epidemiology of human enterovirus 71 at the origin of an epidemic of fatal hand, foot and mouth disease cases in Cambodia.Emerging microbes & infections · 2016Article
- Development and evaluation of a non-ribosomal random PCR and next-generation sequencing based assay for detection and sequencing of hand, foot and mouth disease pathogens.Virology journal · 2016Article
- Detecting spatial-temporal cluster of hand foot and mouth disease in Beijing, China, 2009-2014.BMC infectious diseases · 2016Article
- Therapeutic Use of Native and Recombinant Enteroviruses.Viruses · 2016Review
- Phylodynamics of Enterovirus A71-Associated Hand, Foot, and Mouth Disease in Viet Nam.Journal of virology · 2015Article
Corrections and comments
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Authors and funding
24 authors.
Funding
Abstract
Enterovirus A71 (EV-A71) has emerged as the most important cause of large outbreaks of severe and sometimes fatal hand, foot and mouth disease (HFMD) across the Asia-Pacific region. EV-A71 outbreaks have been associated with (sub)genogroup switches, sometimes accompanied by recombination events. Understanding EV-A71 population dynamics is therefore essential for understanding this emerging infection, and may provide pivotal information for vaccine development. Despite the public health burden of EV-A71, relatively few EV-A71 complete-genome sequences are available for analysis and from limited geographical localities. The availability of an efficient procedure for whole-genome sequencing would stimulate effort to generate more viral sequence data. Herein, we report for the first time the development of a next-generation sequencing based protocol for whole-genome sequencing of EV-A71 directly from clinical specimens. We were able to sequence viruses of subgenogroup C4 and B5, while RNA from culture materials of diverse EV-A71 subgenogroups belonging to both genogroup B and C was successfully amplified. The nature of intra-host genetic diversity was explored in 22 clinical samples, revealing 107 positions carrying minor variants (ranging from 0 to 15 variants per sample). Our analysis of EV-A71 strains sampled in 2013 showed that they all belonged to subgenogroup B5, representing the first report of this subgenogroup in Vietnam. In conclusion, we have successfully developed a high-throughput next-generation sequencing-based assay for whole-genome sequencing of EV-A71 from clinical samples.
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