Molecular detection of the index case of a subclinical Salmonella Kentucky epidemic on a dairy farm.

B J Haley, M Allard, E Brown, E Hovingh, J S Karns, J S van Kessel

Abstract read

In one paragraph

Article in Epidemiology and infection, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it

0cells of the map it votes in

3citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

Genome Sequences ofGenome announcements · 2017
Article
Genome Sequences of Two Salmonella enterica Serovar Kentucky Isolates Recovered from Poultry Carcasses in the United States.Genome announcements · 2016
Article
Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America.PloS one · 2016
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

B J HaleyEnvironmental Microbial and Food Safety Laboratory,Beltsville Agricultural Research Center,USDA-ARS, Beltsville, MD,USA.

M AllardDivision of Microbiology,Center for Food Safety and Nutrition,Food and Drug Administration, College Park, MD,USA.

E BrownDivision of Microbiology,Center for Food Safety and Nutrition,Food and Drug Administration, College Park, MD,USA.

E HovinghAnimal Diagnostic Laboratory,Pennsylvania State University,University Park, PA,USA.

J S KarnsEnvironmental Microbial and Food Safety Laboratory,Beltsville Agricultural Research Center,USDA-ARS, Beltsville, MD,USA.

J S van KesselDivision of Microbiology,Center for Food Safety and Nutrition,Food and Drug Administration, College Park, MD,USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Salmonella enterica commonly colonizes the intestinal tract of cattle and is a leading cause of foodborne illness. A previously described investigation into the prevalence of S. enterica on a dairy farm revealed an 8-year-long asymptomatic S. enterica epidemic caused by serotypes Cerro and Kentucky in the lactating herd. To investigate the source of the S. Kentucky strains, the genomes of two S. Kentucky isolates were sequenced; one collected prior to the epidemic (2004) and one collected during the epidemic (2010). Comparative genomic analysis demonstrated significant polymorphisms between the two strains. PCR primers targeting unique and strain-specific regions were developed, and screening of the archived isolates identified the index case of the asymptomatic S. Kentucky epidemic as a heifer that was raised off-site and transported onto the study farm in 2005. Analysis of isolates collected from all heifers brought onto the farm demonstrated frequent re-introduction of clones of the epidemic strain suggesting transmission of pathogens between farms might occur repeatedly.

Indexed as

AnimalsAsymptomatic InfectionsCattleCattle DiseasesDairyingEpidemicsFemaleGenome, BacterialHumansMolecular EpidemiologyPolymerase Chain ReactionPolymorphism, GeneticSalmonella entericaSalmonella Infections, AnimalUnited StatesSalmonella

Identifiers

PMID25703396

DOI10.1017/S0950268814001289

PMCPMC9507080

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.