ArticleStem cell reports2015
Defined conditions for the isolation and expansion of basal prostate progenitor cells of mouse and human origin.
Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Fresh tissue procurement and preparation for multicompartment and multimodal analysis of the prostate tumor microenvironment.The Prostate · 2022Article
- An Individual Patient's "Body" on Chips-How Organismoid Theory Can Translate Into Your Personal Precision Therapy Approach.Frontiers in medicine · 2021Article
- Engineering organoids.Nature reviews. Materials · 2021Review
- RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development.eLife · 2020Article
- A robust cell culture system for large scale feeder cell-free expansion of human breast epithelial progenitors.Stem cell research & therapy · 2018Article
- Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models.Journal of visualized experiments : JoVE · 2018Article
- Review
- Article
- Multipotent Basal Stem Cells, Maintained in Localized Proximal Niches, Support Directed Long-Ranging Epithelial Flows in Human Prostates.Cell reports · 2017Article
- Prostate organogenesis: tissue induction, hormonal regulation and cell type specification.Development (Cambridge, England) · 2017Review
- Prostate Cancer Stem Cell Markers Drive Progression, Therapeutic Resistance, and Bone Metastasis.Stem cells international · 2017Review
- Current Stem Cell Biomarkers and Their Functional Mechanisms in Prostate Cancer.International journal of molecular sciences · 2016Review
- Basal Progenitors Contribute to Repair of the Prostate Epithelium Following Induced Luminal Anoikis.Stem cell reports · 2016Article
- Protein profile of basal prostate epithelial progenitor cells--stage-specific embryonal antigen 4 expressing cells have enhanced regenerative potential in vivo.Journal of cellular and molecular medicine · 2016Article
- Organoid culture systems for prostate epithelial and cancer tissue.Nature protocols · 2016Article
- The many ways to make a luminal cell and a prostate cancer cell.Endocrine-related cancer · 2015Review
- Isolation and analysis of discreet human prostate cellular populations.Differentiation; research in biological diversityReview
Corrections and comments
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Authors and funding
16 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin(-)SCA-1(+)CD49f(+)TROP2(high) phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin(-)CD49f(+)TROP2(high) PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.