ArticleJournal of assisted reproduction and genetics2015
Scrotal heat stress causes sperm chromatin damage and cysteinyl aspartate-spicific proteinases 3 changes in fertile men.
Article in Journal of assisted reproduction and genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Taurine Attenuates Repeated Heat Stress-Induced Male Reproductive Dysfunction in Mice: Associations with HPG Axis Homeostasis and Hypothalamic Microglial Changes.Reproductive sciences (Thousand Oaks, Calif.) · 2026Article
- Ameliorative Effects of Vitamin C on Heat Stress-Induced Testicular Dysfunction in Wistar Rats.JBRA assisted reproduction · 2026Article
- Serum Biochemical and Gene Expression Changes in the Spermatozoa of Buffalo Bulls Under Heat Stress.Biochemical genetics · 2026Article
- Cryptorchidism and infertility: what do we know so far?Asian journal of andrology · 2026Review
- Article
- The impact of chemical pollution and warming on male fertility: a narrative review by the Special Interest Group "Environment and Fertility" of the Italian Society of Fertility and Sterility and Reproductive Medicine (SIFES-MR).Journal of assisted reproduction and genetics · 2025Review
- The role of the intrinsic pathway of apoptosis in human ejaculated sperm damage under a state of scrotal heat stress.Journal of assisted reproduction and genetics · 2024Article
- Retinoic acid loaded with chitosan nanoparticles improves spermatogenesis in scrotal hyperthermia in mice.Clinical and experimental reproductive medicine · 2023Article
- The Price of Human Evolution: Cancer-Testis Antigens, the Decline in Male Fertility and the Increase in Cancer.International journal of molecular sciences · 2023Review
- Heat Stress as a Barrier to Successful Reproduction and Potential Alleviation Strategies in Cattle.Animals : an open access journal from MDPI · 2023Review
- Negative effect of varicocele on sperm mitochondrial dysfunction: A cross-sectional study.International journal of reproductive biomedicine · 2023Article
- Risk Factors for Testicular Cancer: Environment, Genes and Infections-Is It All?Medicina (Kaunas, Lithuania) · 2023Review
- An Assessment of Men Semen Alterations in SARS-CoV-2: Is Fever the Principal Concern?Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Spatiotemporal trends in human semen quality.Nature reviews. Urology · 2022Review
- Sperm DNA integrity and male infertility: a narrative review and guide for the reproductive physicians.Translational andrology and urology · 2022Review
- The Impact of High Ambient Temperature on Human Sperm Parameters: A Meta-Analysis.Iranian journal of public health · 2022Review
- The Role of Seminal Oxidative Stress Scavenging System in the Pathogenesis of Sperm DNA Damage in Men Exposed and Not Exposed to Genital Heat Stress.International journal of environmental research and public health · 2022Article
- Post-conception heat exposure increases clinically unobserved pregnancy losses.Scientific reports · 2021Article
- Effect of Co-exposure to Heat and Psychological Stressors on Sperm DNA and Semen Parameters.Toxicology reports · 2021Article
- Sperm DNA Fragmentation: A New Guideline for Clinicians.The world journal of men's health · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo observe changes in semen parameters, sperm DNA integrity, chromatin condensation and cysteinyl aspartate-spicific proteinases (Caspase-3) in adult healthy men after scrotal heat stress (SHS).
methodsThe scrotums of 19 healthy male volunteers were exposed to the condition of 40-43 °C SHS belt warming 40 min each day for successive 2 days per week. The course of SHS was continuously 3 months. Routine semen analysis, hypo-osmotic swelling (HOS) test, eosin Y (EY) staining sperm HOS and chromatin dispersion (HOS/SCD) test, HOS and aniline blue (HOS/AB) staining test were carried out before, during and after SHS. The activated Caspase 3 levels of spermatozoa were determined with a microtiter plate reader.
resultsThe mean parameters of sperm concentration, motility and normal morphological sperm were significantly decreased in groups with sperm being collected during SHS 1, 2 and 3 months when compared with those in groups of pre-SHS (P < 0.01). Statistically significant differences of sperm DNA fragmentation, normal sperm membrane and vitality, and Caspase-3 activity were observed between the groups of before SHS and after SHS 3 months and the groups of during SHS 1, 2 and 3 months (P < 0.001). Three months the SHS stopped, various parameters recovered to the level before SHS. Abnormal sperm with HOS/AB and HOS/SCD showed a negatively significant correlation with normal sperm by HOS/EY test, and WBC in semen showed a positively significant correlation with Caspase-3 activity. The percentage of abnormal sperm by using the test of HOS/SCD showed a positively significant correlation with that of HOS/AB.
conclusionsThe continuously constant SHS can impact the semen quality, sperm DNA integrity, chromatin condensation and Caspase-3, and the combination of HOS plus AB test may simultaneously determine the integrity of membrane and chromatin condensation at the same spermatozoon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.