ReviewMethods in molecular biology (Clifton, N.J.)2015
Does super-resolution fluorescence microscopy obsolete previous microscopic approaches to protein co-localization?
Review in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed.
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- Sustained Lysosomal Delivery of Enhanced Cy3-Labeled Acid Nanoparticles Restores Lysosomal pH in Retinal Pigment Epithelial Cells and Astrocytes.bioRxiv : the preprint server for biology · 2025Article
- Closing the multichannel gap through computational reconstruction of interaction in super-resolution microscopy.Patterns (New York, N.Y.) · 2025Review
- Unlocking Intracellular Protein Delivery by Harnessing Polymersomes Synthesized at Microliter Volumes using Photo-PISA.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- The Deposition of Hydroxyapatite Particles Within an Organic Matrix on the Surface of Poly(lactic acid).International journal of molecular sciences · 2024Article
- Trifunctional sphingomyelin derivatives enable nanoscale resolution of sphingomyelin turnover in physiological and infection processes via expansion microscopy.Nature communications · 2024Article
- A-Syn(ful) MAM: A Fresh Perspective on a Converging Domain in Parkinson's Disease.International journal of molecular sciences · 2024Review
- TLR-mediated aggresome-like induced structures comprise antimicrobial peptides and attenuate intracellular bacterial survival.Molecular biology of the cell · 2024Article
- Preparation of Borrelia-Infected Mammalian Cells for Helium Ion Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Defining Assembly Pathways by Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Probing the dynamic crosstalk of lysosomes and mitochondria with structured illumination microscopy.Trends in analytical chemistry : TRAC · 2023Article
- Dual-color core-shell silica nanosystems for advanced super-resolution biomedical imaging.Nanoscale advances · 2023Article
- An In-depth Guide to the Ultrastructural Expansion Microscopy (U-ExM) ofBio-protocol · 2023Article
- Single-molecule tracking of myelin basic protein during oligodendrocyte differentiation.Biological imaging · 2023Article
- The Role of Lipids in CRAC Channel Function.Biomolecules · 2022Review
- Computerized fluorescence microscopy of microbial cells.World journal of microbiology & biotechnology · 2021Review
- Pathologic tau conformer ensembles induce dynamic, liquid-liquid phase separation events at the nuclear envelope.BMC biology · 2021Article
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Innate immune receptor clustering and its role in immune regulation.Journal of cell science · 2021Review
- Nanoscale Infrared Spectroscopy and Chemometrics Enable Detection of Intracellular Protein Distribution.Analytical chemistry · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Conventional microscopy techniques, namely, the confocal microscope or deconvolution processes, are resolution limited to approximately 200-250 nm by the diffraction properties of light as developed by Ernst Abbe in 1873. This diffraction limit is appreciably above the size of most multi-protein complexes, which are typically 20-50 nm in diameter. In the mid-2000s, biophysicists moved beyond the diffraction barrier by structuring the illumination pattern and then applying mathematical principles and algorithms to allow a resolution of approximately 100 nm, sufficient to address protein subcellular co-localization questions. This "breaking" of the diffraction barrier, affording resolution beyond 200 nm, is termed super-resolution microscopy. More recent approaches include single-molecule localization (such as photoactivated localization microscopy (PALM)/stochastic optical reconstruction microscopy (STORM)) and point spread function engineering (such as stimulated emission depletion (STED) microscopy). In this review, we explain basic principles behind currently commercialized super-resolution setups and address advantages and considerations in applying these techniques to protein co-localization in biological systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.