ArticleBiology open2015
Characterisation of detergent-insoluble membranes in pollen tubes of Nicotiana tabacum (L.).
Article in Biology open, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Pollen-expressed methionine synthases in Nicotiana tabacum regulate pollen development and germination.Plant physiology · 2026Article
- Arabidopsis phospholipid modifications mediate cellulase-induced resistance to a fungal peptide antibiotic by imposing cell polarity.The New phytologist · 2026Article
- Inhibition of Sterol Biosynthesis Alters Tubulin Association with Detergent-Insoluble Membranes and Affects Microtubule Organization in Pollen Tubes ofPlants (Basel, Switzerland) · 2025Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- Biosynthesis and the Roles of Plant Sterols in Development and Stress Responses.International journal of molecular sciences · 2022Review
- Evaluation of Adhesive Characteristics ofMicroorganisms · 2021Article
- Facile isolation and analysis of sporopollenin exine from bee pollen.Scientific reports · 2021Article
- Protein Analysis of Pollen Tubes after the Treatments of Membrane Trafficking Inhibitors Gains Insights on Molecular Mechanism Underlying Pollen Tube Polar Growth.The protein journal · 2021Article
- Interactions between lipids and proteins are critical for organization of plasma membrane-ordered domains in tobacco BY-2 cells.Journal of experimental botany · 2018Article
- Retarded germination of Nicotiana tabacum seeds following insertion of exogenous DNA mimics the seed persistent behavior.PloS one · 2017Article
- Emerging roles for microtubules in angiosperm pollen tube growth highlight new research cues.Frontiers in plant science · 2015Article
- Mapping of Membrane Lipid Order in Root Apex Zones of Arabidopsis thaliana.Frontiers in plant science · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen tubes are the vehicle for sperm cell delivery to the embryo sac during fertilisation of Angiosperms. They provide an intriguing model for unravelling mechanisms of growing to extremes. The asymmetric distribution of lipids and proteins in the pollen tube plasma membrane modulates ion fluxes and actin dynamics and is maintained by a delicate equilibrium between exocytosis and endocytosis. The structural constraints regulating polarised secretion and asymmetric protein distribution on the plasma membrane are mostly unknown. To address this problem, we investigated whether ordered membrane microdomains, namely membrane rafts, might contribute to sperm cell delivery. Detergent insoluble membranes, rich in sterols and sphingolipids, were isolated from tobacco pollen tubes. MALDI TOF/MS analysis revealed that actin, prohibitins and proteins involved in methylation reactions and in phosphoinositide pattern regulation are specifically present in pollen tube detergent insoluble membranes. Tubulins, voltage-dependent anion channels and proteins involved in membrane trafficking and signalling were also present. This paper reports the first evidence of membrane rafts in Angiosperm pollen tubes, opening new perspectives on the coordination of signal transduction, cytoskeleton dynamics and polarised secretion.
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