ArticleVirchows Archiv : an international journal of pathology2015
Diagnosing cytomegalovirus in patients with inflammatory bowel disease--by immunohistochemistry or polymerase chain reaction?
Article in Virchows Archiv : an international journal of pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Beyond the first cut: evaluating CMV IHC reliability in serial gastrointestinal biopsy sections.Virchows Archiv : an international journal of pathology · 2026Observational
- Prevalence of Cytomegalovirus in Patients With Newly Diagnosed, Flare-Up, and Acute Severe Ulcerative Colitis.Cureus · 2025Article
- Human Cytomegalovirus and Epstein-Barr Virus Infection in Inflammatory Bowel Disease: Crossing the Diagnostic Barrier for Appropriate Management.Biomedicines · 2025Article
- Characteristics and Clinical Implications of Cytomegalovirus Infection in Patients with Drug-Resistant Ulcerative Colitis Undergoing Colectomy-Data from a Tertiary Referral Center in Poland.Journal of clinical medicine · 2025Article
- Cytomegalovirus in Ulcerative Colitis: An Unwanted "Guest".Pathogens (Basel, Switzerland) · 2024Review
- Cytomegalovirus Infection in Adult Patients with Inflammatory Bowel Disease: A Literature Review.Archives of Iranian medicine · 2024Review
- Cytomegalovirus Infection: An Underrated Target in Inflammatory Bowel Disease Treatment.Journal of clinical medicine · 2023Review
- Review article: Updated management of acute severe ulcerative colitis: From steroids to novel medical strategies.United European gastroenterology journal · 2023Review
- Cytomegalovirus Infections in Patients Treated With Immune Checkpoint Inhibitors for Solid Malignancies.Open forum infectious diseases · 2023Review
- [Cytomegalovirus Infection in Patients with Inflammatory Bowel Disease].The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi · 2022Review
- Cytomegalovirus and Inflammatory Bowel Diseases (IBD) with a Special Focus on the Link with Ulcerative Colitis (UC).Microorganisms · 2020Review
- Current Diagnostic and Therapeutic Approaches to Cytomegalovirus Infections in Ulcerative Colitis Patients Based on Clinical and Basic Research Data.International journal of molecular sciences · 2020Review
- Diagnosis and Management of CMV Colitis.Current infectious disease reports · 2019Review
- CMV Infection in Pediatric IBD.Current gastroenterology reports · 2018Review
- Identification of clinically relevant cytomegalovirus infections in patients with inflammatory bowel disease.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2018Article
- Approach to cytomegalovirus infections in patients with ulcerative colitis.The Korean journal of internal medicine · 2017Review
- Response to Zidar and Tepes.The American journal of gastroenterology · 2017Article
- Cytomegalovirus Reactivation in Ulcerative Colitis: What Can We Learn From Pathology?The American journal of gastroenterology · 2016Article
- Distribution of Cytomegalovirus DNA Load in the Inflamed Colon of Ulcerative Colitis Patients.The American journal of gastroenterology · 2016Article
- Cytomegalovirus and ulcerative colitis: Place of antiviral therapy.World journal of gastroenterology · 2016Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytomegalovirus (CMV) reactivation is a common complication in patients with inflammatory bowel diseases (IBD), particularly in those with steroid-resistant ulcerative colitis. It is usually diagnosed by histopathologic and immunohistochemical examination of the colon biopsy. The introduction of quantitative, real-time polymerase chain reaction (qPCR) has been recommended to improve the sensitivity, but there is little consensus on how to use it. We compared the two methods in samples from resected bowel of patients with IBD. Twelve patients with IBD who had undergone bowel resection were analysed for CMV, using qPCR and immunohistochemistry. In all cases, tissue samples from the base and the edge of ulcers and from uninvolved mucosa were obtained. The highest densities of CMV-positive cells were found in samples from the base of ulcers (immunohistochemistry 0-0.47 positive cells/mm(2); qPCR 10-3809 viral copies/mg) or the edge of ulcers (immunohistochemistry 0.06-0.32 positive cells/mm(2); qPCR 35-1049 viral copies/mg). In samples of uninvolved mucosa, immunohistochemistry was negative, whereas qPCR was either negative or showed very low values (0-3 viral copies/mg). We conclude that both immunohistochemistry and qPCR can be successfully used for diagnosing CMV reactivation in patients with IBD. The base and the edge of ulcers are the optimal sites for endoscopic biopsies. The density of CMV-positive cells was low and their distribution within the colon uneven. It therefore seems that the number of sampled biopsies and/or the number of investigated levels is more important that the choice of diagnostic method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.