ArticleThe Annals of thoracic surgery2015
Ex vivo four-dimensional lung cancer model mimics metastasis.
Article in The Annals of thoracic surgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Animal models of lung cancer: Phenotypic comparison of different animal models of lung cancer and their application in the study of mechanisms.Animal models and experimental medicine · 2025Review
- MMP9 and TYROBP affect the survival of circulating tumor cells in clear cell renal cell carcinoma by adapting to tumor immune microenvironment.Scientific reports · 2023Article
- Tumor Organoid and Spheroid Models for Cervical Cancer.Cancers · 2023Review
- Transcriptional activators YAP/TAZ and AXL orchestrate dedifferentiation, cell fate, and metastasis in human osteosarcoma.Cancer gene therapy · 2021Article
- Selective antagonism of cJun for cancer therapy.Journal of experimental & clinical cancer research : CR · 2020Review
- TPX2 Promotes Metastasis and Serves as a Marker of Poor Prognosis in Non-Small Cell Lung Cancer.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- PI4KIIIβ is a therapeutic target in chromosome 1q-amplified lung adenocarcinoma.Science translational medicine · 2020Article
- Immune cells inhibit the tumor metastasis in the 4D cellular lung model by reducing the number of live circulating tumor cells.Scientific reports · 2018Article
- Acellular and Cellular Lung Model to Study Tumor Metastasis.Journal of visualized experiments : JoVE · 2018Article
- Small cell and non small cell lung cancer form metastasis on cellular 4D lung model.BMC cancer · 2018Article
- Engineering Breast Cancer Microenvironments and 3D Bioprinting.Frontiers in bioengineering and biotechnology · 2018Review
- The Prognostic Role of Circulating Tumor Cells (CTCs) in Lung Cancer.Frontiers in oncology · 2018Review
- SR 11302, an AP-1 Inhibitor, Reduces Metastatic Lesion Formation in Ex Vivo 4D Lung Cancer Model.Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2017Article
- Thy-1Scientific reports · 2017Article
- Targeting KRas-dependent tumour growth, circulating tumour cells and metastasis in vivo by clinically significant miR-193a-3p.Oncogene · 2017Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundWe have developed a four-dimensional (4D) lung cancer model that forms perfusable tumor nodules. We determined if the model could be modified to mimic metastasis.
methodsWe modified the 4D lung cancer model by seeding H1299, A549, or H460 cells through the trachea only to the left lobes of the acellular lung matrix. The model was modified so that the tumor cells can reach the right lobes of the acellular lung matrix only through the pulmonary artery as circulating tumor cells (CTC). We determined the gene expressions of the primary tumor, CTCs, and metastatic lesions using the Human OneArray chip.
resultsAll cell lines formed a primary tumor in the left lobe of the ex vivo 4D lung cancer model. The CTCs were identified in the media and increased over time. All cell lines formed metastatic lesions with H460 forming significantly more metastatic lesions than H1299 and A549 cells. The CTC gene signature predicted poor survival in lung cancer patients. Unique genes were significantly expressed in CTC compared with the primary tumor and metastatic lesion.
conclusionsThe 4D lung cancer model can isolate tumor cells in 3 phases of tumor progression. This 4D lung cancer model may mimic the biology of lung cancer metastasis and may be used to determine its mechanism and potential therapy in the future.
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