ArticleJournal of biomechanics2015
The effect of chemically defined medium on spontaneous calcium signaling of in situ chondrocytes during long-term culture.
Article in Journal of biomechanics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Click Chemistry-Based Quantification of Extracellular Matrix Turnover for Drug Screening and Regenerative Medicine.Annals of biomedical engineering · 2026Article
- Microstructure and matrix-filled lacunae impact mechanical response of temporomandibular joint cartilage under physiological loading.Osteoarthritis and cartilage · 2025Article
- TRPC1 links calcium signaling to cellular senescence in the protection against posttraumatic osteoarthritis.JCI insight · 2024Article
- Triamcinolone acetonide has minimal effect on short- and long-term metabolic activities of cartilage.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2024Article
- Calcium signaling of primary chondrocytes and ATDC5 chondrogenic cells under osmotic stress and mechanical stimulation.Journal of biomechanics · 2022Article
- Review of the Current Research on Fetal Bovine Serum and the Development of Cultured Meat.Food science of animal resources · 2022Review
- Bio-orthogonal Click Chemistry Methods to Evaluate the Metabolism of Inflammatory Challenged Cartilage after Traumatic Overloading.ACS biomaterials science & engineering · 2022Article
- Targeted Activation of G-Protein Coupled Receptor-Mediated CaTissue engineering. Part A · 2022Article
- Substrate stiffness-dependent regulatory volume decrease and calcium signaling in chondrocytes.Acta biochimica et biophysica Sinica · 2022Article
- Spontaneous calcium signaling of cartilage cells: from spatiotemporal features to biophysical modeling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Identification of Chondrocyte Genes and Signaling Pathways in Response to Acute Joint Inflammation.Scientific reports · 2019Article
- Calcium signaling of in situ chondrocytes in articular cartilage under compressive loading: Roles of calcium sources and cell membrane ion channels.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Altered spontaneous calcium signaling of in situ chondrocytes in human osteoarthritic cartilage.Scientific reports · 2017Article
- EGFR signaling is critical for maintaining the superficial layer of articular cartilage and preventing osteoarthritis initiation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Effects of Osmolarity on the Spontaneous Calcium Signaling of In Situ Juvenile and Adult Articular Chondrocytes.Annals of biomedical engineering · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Chemically defined serum-free medium has been shown to better maintain the mechanical integrity of articular cartilage explants than serum-supplemented medium during long-term in vitro culture, but little is known about its effect on cellular mechanisms. We hypothesized that the chemically defined culture medium could regulate the spontaneous calcium signaling of in situ chondrocytes, which may modulate the cellular metabolic activities. Bovine cartilage explants were cultured in chemically defined serum-free or serum-supplemented medium for four weeks. The spontaneous intracellular calcium ([Ca(2+)]i) signaling of in situ chondrocytes was longitudinally measured together along with the biomechanical properties of the explants. The spontaneous [Ca(2+)]i oscillations in chondrocytes were enhanced at the initial exposure of serum-supplemented medium, but were significantly dampened afterwards. In contrast, cartilage explants in chemically defined medium preserved the level of calcium signaling, and showed more responsive cells with higher and more frequent [Ca(2+)]i peaks throughout the four week culture in comparison to those in serum medium. Regardless of the culture medium that the explants were exposed, a positive correlation was detected between the [Ca(2+)]i responsive rate and the stiffness of cartilage (Spearman's rank correlation coefficient=0.762). A stable pattern of [Ca(2+)]i peaks was revealed for each chondrocyte, i.e., the spatiotemporal features of [Ca(2+)]i peaks from a cell were highly consistent during the observation period (15 min). This study showed that the beneficial effect of chemically defined culture of cartilage explants is associated with the spontaneous [Ca(2+)]i signaling of chondrocytes in cartilage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.