ArticleThe Plant cell2015
Small glycosylated lignin oligomers are stored in Arabidopsis leaf vacuoles.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 104 citations in OpenAlex.
- Simultaneous Overexpression of FERULOYL-CoA 6'-HYDROXYLASE 1 and COUMARIN SYNTHASE Leads to Coumarin-Enriched Lignin and Improved Saccharification in Greenhouse- and Field-Grown Poplar.Plant biotechnology journal · 2026Article
- Identification of a Glycosyltransferase-Encoding Gene (Life (Basel, Switzerland) · 2026Article
- Lignin-Carbohydrate Nano-Sized Structures: An Evidence of Intracellular Lignin Biosynthesis?Plants (Basel, Switzerland) · 2026Article
- Physiological roles of lignins - tuning cell wall hygroscopy and biomechanics.The New phytologist · 2025Review
- Factors underlying a latitudinal gradient in the S/G lignin monomer ratio in natural poplar variants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Nature's laboratory: plant metabolic engineering methods using phenylpropanoids as a case study.Biotechnology for biofuels and bioproducts · 2025Review
- Two pathogen-inducible UDP-glycosyltransferases, UGT73C3 and UGT73C4, catalyze the glycosylation of pinoresinol to promote plant immunity in Arabidopsis.Plant communications · 2025Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- Grass lignin: biosynthesis, biological roles, and industrial applications.Frontiers in plant science · 2024Review
- Functional and kinetics of two efficient phenylalanine ammonia lyase from Pyrus bretschneideri.BMC plant biology · 2023Article
- Suppression of the Arabidopsis cinnamoyl-CoA reductase 1-6 intronic T-DNA mutation by epigenetic modification.Plant physiology · 2023Article
- Pinoresinol rescues developmental phenotypes of Arabidopsis phenylpropanoid mutants overexpressingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- QT-GWAS: A novel method for unveiling biosynthetic loci affecting qualitative metabolic traits.Molecular plant · 2023Article
- A broadly conserved fungal alcohol oxidase (AOX) facilitates fungal invasion of plants.Molecular plant pathology · 2023Article
- Comparative Multi-Omics Analysis Reveals Lignin Accumulation Affects Peanut Pod Size.International journal of molecular sciences · 2022Article
- Plant biomechanics and resilience to environmental changes are controlled by specific lignin chemistries in each vascular cell type and morphotype.The Plant cell · 2022Article
- Overexpression of the scopoletin biosynthetic pathway enhances lignocellulosic biomass processing.Science advances · 2022Article
- Identification and characterization of a set of monocot BAHD monolignol transferases.Plant physiology · 2022Article
- Monolignol export by diffusion down a polymerization-induced concentration gradient.The Plant cell · 2022Article
- Developmental changes in lignin composition are driven by both monolignol supply and laccase specificity.Science advances · 2022Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignin is an aromatic polymer derived from the combinatorial coupling of monolignol radicals in the cell wall. Recently, various glycosylated lignin oligomers have been revealed in Arabidopsis thaliana. Given that monolignol oxidation and monolignol radical coupling are known to occur in the apoplast, and glycosylation in the cytoplasm, it raises questions about the subcellular localization of glycosylated lignin oligomer biosynthesis and their storage. By metabolite profiling of Arabidopsis leaf vacuoles, we show that the leaf vacuole stores a large number of these small glycosylated lignin oligomers. Their structural variety and the incorporation of alternative monomers, as observed in Arabidopsis mutants with altered monolignol biosynthesis, indicate that they are all formed by combinatorial radical coupling. In contrast to the common believe that combinatorial coupling is restricted to the apoplast, we hypothesized that the aglycones of these compounds are made within the cell. To investigate this, leaf protoplast cultures were cofed with 13C6-labeled coniferyl alcohol and a 13C4-labeled dimer of coniferyl alcohol. Metabolite profiling of the cofed protoplasts provided strong support for the occurrence of intracellular monolignol coupling. We therefore propose a metabolic pathway involving intracellular combinatorial coupling of monolignol radicals, followed by oligomer glycosylation and vacuolar import, which shares characteristics with both lignin and lignan biosynthesis.
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