ArticleFrontiers in plant science2015
Characterization of reference genes for RT-qPCR in the desert moss Syntrichia caninervis in response to abiotic stress and desiccation/rehydration.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 50 citations in OpenAlex.
- Comparative Analysis of Full-Length Reference Gene Stability inPlants (Basel, Switzerland) · 2026Article
- Selection and Validation of Reference Genes for RT-qPCR in Protonemal Tissue of the Desiccation-Tolerant MossPlants (Basel, Switzerland) · 2025Article
- A simple, highly efficientaBIOTECH · 2024Article
- Arbuscular mycorrhiza augments aluminum tolerance in white clover (Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Genome-Wide Characterization and Expression Analysis of Transcription Factor Families in Desert MossInternational journal of molecular sciences · 2023Article
- Genome-Wide Characterization and Expression Profiling of ABA Biosynthesis Genes in a Desert MossPlants (Basel, Switzerland) · 2023Article
- Transcriptional profiling analysis providing insights into desiccation tolerance mechanisms of the desert mossFrontiers in plant science · 2023Article
- Impact of the Arbuscular Mycorrhizal FungusJournal of fungi (Basel, Switzerland) · 2022Article
- The ABI3 Transcription Factor Interaction and Antagonism with Ubiquitin E3 Ligase ScPRT1 inGenes · 2022Article
- The DREB A-5 Transcription Factor ScDREB5 FromFrontiers in plant science · 2022Article
- Selection and Verification of Appropriate Reference Genes for Expression Normalization inGenes · 2021Article
- Selection and Validation of Reference Genes for Normalisation of Gene Expression in Glehnia littoralis.Scientific reports · 2020Article
- Ascorbate Peroxidase of Moss Dicranum scoparium: Gene Identification and Enzyme Activity.Doklady. Biochemistry and biophysics · 2019Article
- Article
- Validation of Reference Genes for Gene Expression Normalization in RAW264.7 Cells under Different Conditions.BioMed research international · 2019Article
- Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization ofBioMed research international · 2018Article
- Identification of reference genes for RT-qPCR in the Antarctic moss Sanionia uncinata under abiotic stress conditions.PloS one · 2018Article
- Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Salix matsudana under different abiotic stresses.Scientific reports · 2017Article
- Stability evaluation of reference genes for gene expression analysis by RT-qPCR in soybean under different conditions.PloS one · 2017Article
- Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress.Plant methods · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Syntrichia caninervis is the dominant bryophyte of the biological soil crusts found in the Gurbantunggut desert. The extreme desert environment is characterized by prolonged drought, temperature extremes, high radiation and frequent cycles of hydration and dehydration. S. caninervis is an ideal organism for the identification and characterization of genes related to abiotic stress tolerance. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) expression analysis is a powerful analytical technique that requires the use of stable reference genes. Using available S. caninervis transcriptome data, we selected 15 candidate reference genes and analyzed their relative expression stabilities in S. caninervis gametophores exposed to a range of abiotic stresses or a hydration-desiccation-rehydration cycle. The programs geNorm, NormFinder, and RefFinder were used to assess and rank the expression stability of the 15 candidate genes. The stability ranking results of reference genes under each specific experimental condition showed high consistency using different algorithms. For abiotic stress treatments, the combination of two genes (α-TUB2 and CDPK) were sufficient for accurate normalization. For the hydration-desiccation-rehydration process, the combination of two genes (α-TUB1 and CDPK) were sufficient for accurate normalization. 18S was among the least stable genes in all of the experimental sets and was unsuitable as reference gene in S. caninervis. This is the first systematic investigation and comparison of reference gene selection for RT-qPCR work in S. caninervis. This research will facilitate gene expression studies in S. caninervis, related moss species from the Syntrichia complex and other mosses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.