ArticleFrontiers in cellular neuroscience2015
Bruchpilot and Synaptotagmin collaborate to drive rapid glutamate release and active zone differentiation.
Article in Frontiers in cellular neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Article
- Active Zone: Linking Resolution Levels of Microscopic Modalities.Advances in neurobiology · 2026Review
- Octopamine signaling regulates the intracellular pattern of the presynaptic active zone scaffold within Drosophila mushroom body neurons.PLoS biology · 2025Article
- Balanced synapse-to-synapse short-term plasticity ensures constant transmitter release.Current biology : CB · 2025Article
- Article
- Neto proteins differentially modulate the gating properties ofbioRxiv : the preprint server for biology · 2024Article
- Nanoscaled RIM clustering at presynaptic active zones revealed by endogenous tagging.Life science alliance · 2023Article
- Bruchpiloting synaptic connections: Egfr in autophagy-mediated neuronal circuit development.Autophagy · 2023Article
- A leaky integrate-and-fire computational model based on the connectome of the entire adultbioRxiv : the preprint server for biology · 2023Article
- Single-Molecule Localization Microscopy of Presynaptic Active Zones inInternational journal of molecular sciences · 2023Article
- Endogenous tagging of Unc-13 reveals nanoscale reorganization at active zones during presynaptic homeostatic potentiation.Frontiers in cellular neuroscience · 2022Article
- Glutamatergic Synapse Dysfunction inFrontiers in molecular neuroscience · 2022Article
- Separation of presynaptic CaProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Super-resolution microscopy for analyzing neuromuscular junctions and synapses.Neuroscience letters · 2020Review
- Homeostatic scaling of active zone scaffolds maintains global synaptic strength.The Journal of cell biology · 2019Article
- Complexin cooperates with Bruchpilot to tether synaptic vesicles to the active zone cytomatrix.The Journal of cell biology · 2019Article
- Characterization of developmental and molecular factors underlying release heterogeneity ateLife · 2018Article
- Synapse-specific and compartmentalized expression of presynaptic homeostatic potentiation.eLife · 2018Article
- Presynaptic morphogenesis, active zone organization and structural plasticity in Drosophila.Current opinion in neurobiology · 2017Review
- Input-Specific Plasticity and Homeostasis at the Drosophila Larval Neuromuscular Junction.Neuron · 2017Article
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7 authors.
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Abstract
The active zone (AZ) protein Bruchpilot (Brp) is essential for rapid glutamate release at Drosophila melanogaster neuromuscular junctions (NMJs). Quantal time course and measurements of action potential-waveform suggest that presynaptic fusion mechanisms are altered in brp null mutants (brp(69) ). This could account for their increased evoked excitatory postsynaptic current (EPSC) delay and rise time (by about 1 ms). To test the mechanism of release protraction at brp(69) AZs, we performed knock-down of Synaptotagmin-1 (Syt) via RNAi (syt(KD) ) in wildtype (wt), brp(69) and rab3 null mutants (rab3(rup) ), where Brp is concentrated at a small number of AZs. At wt and rab3(rup) synapses, syt(KD) lowered EPSC amplitude while increasing rise time and delay, consistent with the role of Syt as a release sensor. In contrast, syt(KD) did not alter EPSC amplitude at brp(69) synapses, but shortened delay and rise time. In fact, following syt(KD) , these kinetic properties were strikingly similar in wt and brp(69) , which supports the notion that Syt protracts release at brp(69) synapses. To gain insight into this surprising role of Syt at brp(69) AZs, we analyzed the structural and functional differentiation of synaptic boutons at the NMJ. At 'tonic' type Ib motor neurons, distal boutons contain more AZs, more Brp proteins per AZ and show elevated and accelerated glutamate release compared to proximal boutons. The functional differentiation between proximal and distal boutons is Brp-dependent and reduced after syt(KD) . Notably, syt(KD) boutons are smaller, contain fewer Brp positive AZs and these are of similar number in proximal and distal boutons. In addition, super-resolution imaging via dSTORM revealed that syt(KD) increases the number and alters the spatial distribution of Brp molecules at AZs, while the gradient of Brp proteins per AZ is diminished. In summary, these data demonstrate that normal structural and functional differentiation of Drosophila AZs requires concerted action of Brp and Syt.
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