ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2015
Direct susceptibility testing by disk diffusion on clinical samples: a rapid and accurate tool for antibiotic stewardship.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Systematic review and meta-analysis of antimicrobial resistant bacteria in free-ranging wild mammals.BMC veterinary research · 2025Pooled it
- A mobile based antimicrobial susceptibility testing device - prototype development and validation.The Indian journal of medical research · 2026Article
- Rapid direct disk diffusion testing for antibiotic resistance in urinary tract infections: a bacterial concentration-adjusted approach.Microbiology spectrum · 2025Article
- Review
- Reviewing the complexities of bacterial biocide susceptibility and in vitro biocide adaptation methodologies.npj antimicrobials and resistance · 2025Review
- Probiotic Antimicrobial Evaluation Via Real-Time Profiling of Bacterial Cell Proliferation Using Stochastic Kinetics.ACS sensors · 2025Article
- Parallel Detection of the Unamplified Carbapenem Resistance GenesBiosensors · 2025Article
- Evaluation of Direct Antimicrobial Susceptibility Testing from Positive Flagged Blood Cultures in Sepsis Patients.Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine · 2024Article
- Methodological Approach to Identify and Expand the Volume of Antimicrobial Resistance (AMR) Data in the Human Health Sector in Low- and Middle-Income Countries in Asia: Implications for Local and Regional AMR Surveillance Systems Strengthening.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2023Article
- Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenicSensors & diagnostics · 2023Article
- The African Wastewater Resistome: Identifying Knowledge Gaps to Inform Future Research Directions.Antibiotics (Basel, Switzerland) · 2023Review
- Effective or Harmful-Evaluation of Locally Applied Antibiotics on Adipose Tissue during Lipofilling to the Breast-An In Vitro Study.International journal of molecular sciences · 2023Article
- Analysis and management of pathogens isolated from patients with complicated facial lacerations and abrasions.International wound journal · 2023Article
- Green synthesis of silver nanoparticles usingHeliyon · 2022Article
- Direct Urine Resistance Detection Using VITEK 2.Antibiotics (Basel, Switzerland) · 2022Article
- Phenotypic and genotypic within-host diversity of Pseudomonas aeruginosa urinary isolates.Scientific reports · 2022Article
- Isolation, Identification, and Genetic Characterization of Antibiotic Resistance ofJournal of tropical medicine · 2022Article
- Evaluation of the Performance of Direct Susceptibility Test by VITEK-2 from Positively Flagged Blood Culture Broth for Gram-Negative Bacilli.Journal of laboratory physicians · 2021Article
- Article
- Antibacterial and Anticancer Activities of Fenugreek Seed Extract.Asian Pacific journal of cancer prevention : APJCP · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared the accuracy of direct susceptibility testing (DST) with conventional antimicrobial susceptibility testing (AST), both using disk diffusion, on clinical samples. A total of 123 clinical samples (respiratory tract samples, urine, vaginal and abdominal abscess discharges, bile fluid and a haematoma punctate) were selected on various indications; direct inoculation on Mueller-Hinton agar and antibiotic paper disks were applied. In parallel, standard culture, identification and AST on the colonies grown overnight was executed. Both AST and DST were interpreted after identification of the isolates. The results from both AST and DST for 11 antibiotics tested on 97 samples with Gram-negative rods showed 93.4 % total agreement, 1.6 % minor discordances, 4.6 % major discordances and 0.4 % very major discordances. Analysing the discordant results, DST predominantly resulted in more resistant isolates than AST. This was mostly due to the presence of resistant mutants or an additional isolate. The remaining discordances were seen for isolates with inhibition zones close to the clinical breakpoint. For the 26 samples yielding staphylococci, a total agreement of 100 % was observed for the nine antibiotics tested. Overall, the highest percentage of discordant results occurred for the β-lactam antibiotics amoxicillin-clavulanate (13.4 %) and cefuroxime (12.4 %). When used selectively and interpreted carefully, DST on clinical samples is potentially very useful in the management of critically ill patients, as the time to results is shortened by approximately 24 h. However, we recommend to communicate results with reservations and confirm by conventional AST.
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