ArticlePlant cell reports2015
Inside out: high-efficiency plant regeneration and Agrobacterium-mediated transformation of upland and lowland switchgrass cultivars.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed.
- Genetic transformation of forage crops: comparative barriers, evidence, and emerging strategies.Plant cell reports · 2026Review
- Toward improving multiomic research in understudied cereals.Nature genetics · 2025Review
- Article
- Genome-wide characterization of LBD transcription factors in switchgrass (Panicum virgatum L.) and the involvement of PvLBD12 in salt tolerance.Plant cell reports · 2023Article
- Establishment of an EfficientInternational journal of molecular sciences · 2023Article
- Optimization of callus induction and proliferation ofFrontiers in plant science · 2022Article
- Establishment of an efficientMolecular breeding : new strategies in plant improvement · 2021Article
- MiR396-GRF module associates with switchgrass biomass yield and feedstock quality.Plant biotechnology journal · 2021Article
- Genetic Transformation of Recalcitrant Upland Switchgrass Using Morphogenic Genes.Frontiers in plant science · 2021Article
- Genetic Transformation of Apomictic Grasses: Progress and Constraints.Frontiers in plant science · 2021Review
- ADP-ribosylation factors improve biomass yield and salinity tolerance in transgenic switchgrass (Panicum virgatum L.).Plant cell reports · 2020Article
- Heterologous expression of a chimeric gene, OsDST-SRDX, enhanced salt tolerance of transgenic switchgrass (Panicum virgatum L.).Plant cell reports · 2020Article
- Robust and ReproducibleFrontiers in plant science · 2020Article
- Proline Biosynthesis Enzyme Genes Confer Salt Tolerance to Switchgrass (Frontiers in plant science · 2020Article
- MiR319 mediated salt tolerance by ethylene.Plant biotechnology journal · 2019Article
- Embryogenic cell suspensions for high-capacity genetic transformation and regeneration of switchgrass (Biotechnology for biofuels · 2019Article
- Article
- Overexpression of gene encoding the key enzyme involved in proline-biosynthesis (PuP5CS) to improve salt tolerance in switchgrass (Panicum virgatum L.).Plant cell reports · 2018Article
- Overexpression of ovine AANAT and HIOMT genes in switchgrass leads to improved growth performance and salt-tolerance.Scientific reports · 2017Article
- Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure forPlant methods · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageSelection of pre-embryogenic callus from a core structure from mature seed-derived callus is the key for high-efficiency plant regeneration and transformation of switchgrass different cultivars. Switchgrass (Panicum virgatum L.) has been identified as a dedicated biofuel crop. For its trait improvement through biotechnological approaches, we have developed a highly efficient plant regeneration and genetic transformation protocol for both lowland and upland cultivars. We identified and separated a pre-embryogenic "core" structure from the seed-derived callus, which often leads to development of highly regenerative type II calluses. From the type II callus, plant regeneration rate of lowland cultivars Alamo and Performer reaches 95%, and upland cultivars Blackwell and Dacotah, 50 and 76%, respectively. The type II callus was also amenable for Agrobacterium-mediated transformation. Transformation efficiency of 72.8% was achieved for lowland cultivar Alamo, and 8.0% for upland cultivar Dacotah. PCR, Southern blot and GUS staining assays were performed to verify the transgenic events. High regenerative callus lines could be established in 3 months, and transgenic plants could be obtained in 2 months after Agrobacterium infection. To our knowledge, this is the first report on successful plant regeneration and recovery of transgenic plants from upland switchgrass cultivars by Agrobacterium-mediated transformation. The method presented here could be helpful in breaking through the bottleneck of regeneration and transformation of lowland and upland switchgrass cultivars and probably other recalcitrant grass crops.
Indexed as
Identifiers
25698105What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.