ArticleJournal of experimental botany2015
A defence-related Olea europaea β-glucosidase hydrolyses and activates oleuropein into a potent protein cross-linking agent.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 63 citations in OpenAlex.
- Comparative transcriptomic and co-expression analyses enable the discovery of key enzymes responsible for oleuropein biosynthesis in olive (Olea europaea).Plant communications · 2026Article
- Discovery of iridoid cyclase completes the iridoid pathway in asterids.Nature plants · 2025Article
- Antidiabetic potential of vanadium complexes combined with olive leaf extracts: a viable approach to reduce metal toxicity.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Article
- Foliar Nutrition Strategies for Enhancing Phenolic and Amino Acid Content in Olive Leaves.Plants (Basel, Switzerland) · 2024Article
- Oleocanthal and Oleacein from Privet Leaves: An Alternative Source for High-Value Extra Virgin Olive Oil Bioactives.International journal of molecular sciences · 2024Article
- Microbial and Biochemical Profile of Different Types of Greek Table Olives.Foods (Basel, Switzerland) · 2023Article
- The Infestation of Olive Fruits byMolecules (Basel, Switzerland) · 2022Article
- Scions impact biomass allocation and root enzymatic activity of rootstocks in grafted melon and watermelon plants.Frontiers in plant science · 2022Article
- Functional Characterization of Genes Coding for NovelFrontiers in plant science · 2022Article
- Activation of specific bitter taste receptors by olive oil phenolics and secoiridoids.Scientific reports · 2021Article
- Genome Wide MeDIP-Seq Profiling of Wild and Cultivated Olives Trees Suggests DNA Methylation Fingerprint on the Sensory Quality of Olive Oil.Plants (Basel, Switzerland) · 2021Article
- Alternative splicing creates a pseudo-strictosidine β-d-glucosidase modulating alkaloid synthesis in Catharanthus roseus.Plant physiology · 2021Article
- Optimization of extracellular ethanol-tolerant β-glucosidase production from a newly isolated Aspergillus sp. DHE7 via solid state fermentation using jojoba meal as substrate: purification and biochemical characterization for biofuel preparation.Journal, genetic engineering & biotechnology · 2021Article
- Polyphenols in the Mediterranean Diet: From Dietary Sources to microRNA Modulation.Antioxidants (Basel, Switzerland) · 2021Review
- Silencing of Oleuropein β-Glucosidase Abolishes the Biosynthetic Capacity of Secoiridoids in Olives.Frontiers in plant science · 2021Article
- Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation amongInternational journal of molecular sciences · 2020Article
- Management of Plant Physiology with Beneficial Bacteria to Improve Leaf Bioactive Profiles and Plant Adaptation under Saline Stress inFoods (Basel, Switzerland) · 2020Article
- Article
- Two novel methylesterases from Olea europaea contribute to the catabolism of oleoside-type secoiridoid esters.Planta · 2019Article
- Identification and characterization of a novel β-glucosidase via metagenomic analysis of Bursaphelenchus xylophilus and its microbial flora.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oleuropein, the major secoiridoid compound in olive, is involved in a sophisticated two-component defence system comprising a β-glucosidase enzyme that activates oleuropein into a toxic glutaraldehyde-like structure. Although oleuropein deglycosylation studies have been monitored extensively, an oleuropein β-glucosidase gene has not been characterized as yet. Here, we report the isolation of OeGLU cDNA from olive encoding a β-glucosidase belonging to the defence-related group of terpenoid-specific glucosidases. In planta recombinant protein expression assays showed that OeGLU deglycosylated and activated oleuropein into a strong protein cross-linker. Homology and docking modelling predicted that OeGLU has a characteristic (β/α)8 TIM barrel conformation and a typical construction of a pocket-shaped substrate recognition domain composed of conserved amino acids supporting the β-glucosidase activity and non-conserved residues associated with aglycon specificity. Transcriptional analysis in various olive organs revealed that the gene was developmentally regulated, with its transcript levels coinciding well with the spatiotemporal patterns of oleuropein degradation and aglycon accumulation in drupes. OeGLU upregulation in young organs reflects its prominent role in oleuropein-mediated defence system. High gene expression during drupe maturation implies an additional role in olive secondary metabolism, through the degradation of oleuropein and reutilization of hydrolysis products.
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