ArticleThe Journal of biological chemistry2015
Conformational changes leading to T7 DNA delivery upon interaction with the bacterial receptor.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 82 citations in OpenAlex.
- Article
- A synthetic cell phage cycle.Nature communications · 2025Article
- Systematic genome-wide mapping of host determinants of bacteriophage infectivity.Cell systems · 2025Article
- Targeting multidrug-resistant bacteria with genetic-information-free protein-only phages.bioRxiv : the preprint server for biology · 2025Article
- Synthetic and Functional Engineering of Bacteriophages: Approaches for Tailored Bactericidal, Diagnostic, and Delivery Platforms.Molecules (Basel, Switzerland) · 2025Review
- Amino acid residues in the tail fiber differentiate the host specificity ofJournal of virology · 2025Article
- Engineering bacteriophages through deep mining of metagenomic motifs.Science advances · 2025Article
- Completing the BASEL phage collection to unlock hidden diversity for systematic exploration of phage-host interactions.PLoS biology · 2025Article
- The structure of Shigella virus Sf14 reveals the presence of two decoration proteins and two long tail fibers.Communications biology · 2025Article
- Conformational changes in and translocation of small proteins: insights into the ejection mechanism of podophages.Journal of virology · 2025Article
- Engineering Phages to Fight Multidrug-Resistant Bacteria.Chemical reviews · 2025Review
- Viral Genome Delivery Across Bacterial Cell Surfaces.Annual review of microbiology · 2024Review
- Principles, Methods, and Real-Time Applications of Bacteriophage-Based Pathogen Detection.Molecular biotechnology · 2024Review
- Article
- PHEIGES: all-cell-free phage synthesis and selection from engineered genomes.Nature communications · 2024Article
- A hybrid and poly-polish workflow for the complete and accurate assembly of phage genomes: a case study of ten przondoviruses.Microbial genomics · 2023Article
- Resistance ofMicrobiology spectrum · 2023Article
- Deciphering Bacteriophage T5 Host Recognition Mechanism and Infection Trigger.Journal of virology · 2023Article
- Host genetic requirements for DNA release of lactococcal phage TP901-1.Microbial biotechnology · 2022Article
- Viral Ejection Proteins: Mosaically Conserved, Conformational Gymnasts.Microorganisms · 2022Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The majority of bacteriophages protect their genetic material by packaging the nucleic acid in concentric layers to an almost crystalline concentration inside protein shells (capsid). This highly condensed genome also has to be efficiently injected into the host bacterium in a process named ejection. Most phages use a specialized complex (often a tail) to deliver the genome without disrupting cell integrity. Bacteriophage T7 belongs to the Podoviridae family and has a short, non-contractile tail formed by a tubular structure surrounded by fibers. Here we characterize the kinetics and structure of bacteriophage T7 DNA delivery process. We show that T7 recognizes lipopolysaccharides (LPS) from Escherichia coli rough strains through the fibers. Rough LPS acts as the main phage receptor and drives DNA ejection in vitro. The structural characterization of the phage tail after ejection using cryo-electron microscopy (cryo-EM) and single particle reconstruction methods revealed the major conformational changes needed for DNA delivery at low resolution. Interaction with the receptor causes fiber tilting and opening of the internal tail channel by untwisting the nozzle domain, allowing release of DNA and probably of the internal head proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.