ArticleThe Journal of biological chemistry2015
Myeloperoxidase-dependent lipid peroxidation promotes the oxidative modification of cytosolic proteins in phagocytic neutrophils.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- NADPH oxidase-dependent free radical generation and protein adduct formation in neutrophils.RSC advances · 2024Article
- Post-translational modifications on the metal-sequestering protein calprotectin.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2023Review
- Effects of dietary supplementation of vitamin-E and commercial probiotics on the innate immunity ofFish and shellfish immunology reports · 2021Article
- Myeloperoxidase-Derived 2-Chlorohexadecanal Is Generated in Mouse Heart during Endotoxemia and Induces Modification of Distinct Cardiomyocyte Protein Subsets In Vitro.International journal of molecular sciences · 2020Article
- The Imbalance among Oxidative Biomarkers and Antioxidant Defense Systems in Thromboangiitis Obliterans (Winiwarter-Buerger Disease).Journal of clinical medicine · 2020Article
- Oxidative cross-linking of calprotectin occurs in vivo, altering its structure and susceptibility to proteolysis.Redox biology · 2019Article
- The Transcription Factor ArcA ModulatesFrontiers in microbiology · 2019Article
- Modulation of Innate Immunity by G-CSF and Inflammatory Response by LBPK95A Improves the Outcome of Sepsis in a Rat Model.Journal of immunology research · 2018Article
- Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid.Oxidative medicine and cellular longevity · 2018Article
- Expression, Purification, and Antimicrobial Activity of S100A12.Journal of visualized experiments : JoVE · 2017Article
- Endotoxin promotes neutrophil hierarchical chemotaxis via the p38-membrane receptor pathway.Oncotarget · 2016Article
- Pleiotropic effects of 4-hydroxynonenal on oxidative burst and phagocytosis in neutrophils.Redox biology · 2016Article
- Neutrophil-generated oxidative stress and protein damage in Staphylococcus aureus.Pathogens and disease · 2016Review
- Assessment of electrophile damage in a human brain endothelial cell line utilizing a clickable alkyne analog of 2-chlorohexadecanal.Free radical biology & medicine · 2016Article
- The Human Antimicrobial Protein Calgranulin C Participates in Control of Helicobacter pylori Growth and Regulation of Virulence.Infection and immunity · 2015Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phagocytic neutrophils generate reactive oxygen species to kill microbes. Oxidant generation occurs within an intracellular phagosome, but diffusible species can react with the neutrophil and surrounding tissue. To investigate the extent of oxidative modification, we assessed the carbonylation of cytosolic proteins in phagocytic neutrophils. A 4-fold increase in protein carbonylation was measured within 15 min of initiating phagocytosis. Carbonylation was dependent on NADPH oxidase and myeloperoxidase activity and was inhibited by butylated hydroxytoluene and Trolox, indicating a role for myeloperoxidase-dependent lipid peroxidation. Proteomic analysis of target proteins revealed significant carbonylation of the S100A9 subunit of calprotectin, a truncated form of Hsp70, actin, and hemoglobin from contaminating erythrocytes. The addition of the reactive aldehyde 4-hydroxynonenal (HNE) caused carbonylation, and HNE-glutathione adducts were detected in the cytosol of phagocytic neutrophils. The post-translational modification of neutrophil proteins will influence the functioning and fate of these immune cells in the period following phagocytic activation, and provides a marker of neutrophil activation during infection and inflammation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.