ArticlePlanta2015
High-throughput sequencing reveals differential expression of miRNAs in tomato inoculated with Phytophthora infestans.
Article in Planta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 82 citations in OpenAlex.
- Article
- The lncRNA20718-miR6022-RLPs module regulates tomato resistance to Phytophthora infestans.Plant cell reports · 2024Article
- Review
- Identification of Tomato microRNAs in Late Response toInternational journal of molecular sciences · 2024Article
- Differential expression of microRNAs in response toFrontiers in plant science · 2024Article
- Identification of nitric oxide mediated defense signaling and its microRNA mediated regulation duringPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Characterization of microRNAs and Target Genes inPlants (Basel, Switzerland) · 2023Article
- Identification of miRNAs Involving Potato-Plants (Basel, Switzerland) · 2023Article
- Recent Developments in the Application of Plant Growth-Promoting Drought Adaptive Rhizobacteria for Drought Mitigation.Plants (Basel, Switzerland) · 2022Article
- Systematic identification of miRNA-regulatory networks unveils their potential roles in sugarcane response to Sorghum mosaic virus infection.BMC plant biology · 2022Article
- miRNAs and lncRNAs in tomato: Roles in biotic and abiotic stress responses.Frontiers in plant science · 2022Review
- Function identification of miR394 in tomato resistance to Phytophthora infestans.Plant cell reports · 2021Article
- Transcriptomic and Metabolomic Approaches Deepen Our Knowledge of Plant-Endophyte Interactions.Frontiers in plant science · 2021Review
- Genome-Wide Identification and Characterization of Potato Long Non-coding RNAs Associated WithFrontiers in plant science · 2021Article
- miR159 Represses a Constitutive Pathogen Defense Response in Tobacco.Plant physiology · 2020Article
- Unique miRNAs and their targets in tomato leaf responding to combined drought and heat stress.BMC plant biology · 2020Article
- Article
- Differential expression of microRNAs in tomato leaves treated with different light qualities.BMC genomics · 2020Article
- Construction of ceRNA Coexpression Network and Screening of Molecular Targets in Colorectal Cancer.Disease markers · 2020Article
- Identification of Appropriate Reference Genes for Normalizing miRNA Expression in Citrus Infected byGenes · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionThe characterization and compare expression profiling of the miRNA transcriptome lay a solid foundation for unraveling the complex miRNA-mediated regulatory network in tomato resistance mechanisms against LB. MicroRNAs (miRNAs) are a class of small endogenous non-coding RNAs with 20-24 nt. They have been identified in many plants with their diverse regulatory roles in biotic stresses. The knowledge, that miRNAs regulate late blight (LB), caused by Phytophthora infestans, is rather limited. In this study, we used miRNA-Seq to investigate the miRNA expression difference between the tomatoes treated with and without P. infestans. A total of 42,714,516 raw reads were generated from two small RNA libraries by high-throughput sequencing. Finally, 207 known miRNAs and 67 new miRNAs were obtained. The differential expression profile of miRNAs in tomato was further analyzed with twofold change (P value ≤0.01). A total of 70 miRNAs were manifested to change significantly in samples treated with P. infestans, including 50 down-regulated miRNAs and 20 up-regulated miRNAs. Moreover, a total of 73 target genes were acquired for 28 differentially expressed miRNAs by psRNATarget analysis. By enrichment pathway analysis of target genes, plant-pathogen interaction was the most highly relevant pathway which played an important role in disease defense. In addition, 30 miRNAs were selected for qRT-PCR to validate their expression patterns. The expression patterns for targets of miR6027, miR5300, miR476b, miR159a, miR164a and miRn13 were selectively examined, and the results showed that there was a negative correlation on the expression patterns between miRNAs and their targets. The targets have previously been reported to be related with plant immune and involved in plant-pathogen interaction pathway in this study, suggesting these miRNAs might act as regulators in process of tomato resistance against P. infestans. These discoveries will provide us useful information to explain tomato resistance mechanisms against LB.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.