ArticleJournal of neurophysiology2015
Isoflurane abolishes spontaneous firing of serotonin neurons and masks their pH/CO₂ chemosensitivity.
Article in Journal of neurophysiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Neural basis for mutant ATAXIN-1 induced respiratory dysfunction in mouse models of spinocerebellar ataxia type 1.Neurobiology of disease · 2026Article
- Interactions between Arousal State and COThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Optogenetic activation of dorsal raphe serotonin neurons induces brain-wide activation.Nature communications · 2024Article
- Whole-brain analysis of CObioRxiv : the preprint server for biology · 2023Article
- Criteria for central respiratory chemoreceptors: experimental evidence supporting current candidate cell groups.Frontiers in physiology · 2023Review
- A CRISPR toolbox for generating intersectional genetic mouse models for functional, molecular, and anatomical circuit mapping.BMC biology · 2022Article
- Differential modulation of active expiration during hypercapnia by the medullary raphe in unanesthetized rats.Pflugers Archiv : European journal of physiology · 2020Article
- Perinatal Nicotine Reduces Chemosensitivity of Medullary 5-HT Neurons after Maturation in Culture.Neuroscience · 2020Article
- Isoflurane inhibits a Kir4.1/5.1-like conductance in neonatal rat brainstem astrocytes and recombinant Kir4.1/5.1 channels in a heterologous expression system.Journal of neurophysiology · 2020Article
- Arterial blood sampling in male CD-1 and C57BL/6J mice with 1% isoflurane is similar to awake mice.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
- In vivo biased agonism at 5-HTNeuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2018Article
- KCC2-dependent Steady-state Intracellular Chloride Concentration and pH in Cortical Layer 2/3 Neurons of Anesthetized and Awake Mice.Frontiers in cellular neuroscience · 2018Article
- Isoflurane, ketamine-xylazine, and urethane markedly alter breathing even at subtherapeutic doses.Journal of neurophysiology · 2017Article
- Role of A5 noradrenergic neurons in the chemoreflex control of respiratory and sympathetic activities in unanesthetized conditions.Neuroscience · 2017Article
- Effect of Isoflurane Anesthesia on Circadian Metabolism and Physiology in Rats.Comparative medicine · 2017Article
- Activity ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Impaired central respiratory chemoreflex in an experimental genetic model of epilepsy.The Journal of physiology · 2017Article
- Article
- Improved assessment of outcomes following transient global cerebral ischemia in mice.Experimental brain research · 2016Article
- Impaired Serotonergic Brainstem Function during and after Seizures.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Serotonin (5-hydroxytryptamine, 5-HT) neurons from the mouse and rat rostral medulla are stimulated by increased CO2 when studied in culture or brain slices. However, the response of 5-HT neurons has been variable when animals are exposed to hypercapnia in vivo. Here we examined whether halogenated inhalational anesthetics, which activate TWIK-related acid-sensitive K(+) (TASK) channels, could mask an effect of CO2 on 5-HT neurons. During in vivo plethysmography in mice, isoflurane (1%) markedly reduced the hypercapnic ventilatory response (HCVR) by 78-96% depending upon mouse strain and ambient temperature. In a perfused rat brain stem preparation, isoflurane (1%) reduced or silenced spontaneous firing of medullary 5-HT neurons in situ and abolished their responses to elevated perfusate Pco2. In dissociated cell cultures, isoflurane (1%) hyperpolarized 5-HT neurons by 6.52 ± 3.94 mV and inhibited spontaneous firing. A subsequent decrease in pH from 7.4 to 7.2 depolarized neurons by 4.07 ± 2.10 mV, but that was insufficient to reach threshold for firing. Depolarizing current restored baseline firing and the firing frequency response to acidosis, indicating that isoflurane did not block the underlying mechanisms mediating chemosensitivity. These results demonstrate that isoflurane masks 5-HT neuron chemosensitivity in vitro and in situ and markedly decreases the HCVR in vivo. The use of this class of anesthetic has a particularly potent inhibitory effect on chemosensitivity of 5-HT neurons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.