ArticleThe Journal of cell biology1989
Acanthamoeba actin and profilin can be cross-linked between glutamic acid 364 of actin and lysine 115 of profilin.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 117 citations in OpenAlex.
- Interaction of profilin with the barbed end of actin filaments.Biochemistry · 2013Article
- Depolymerization of cortical actin inhibits UT-A1 urea transporter endocytosis but promotes forskolin-stimulated membrane trafficking.American journal of physiology. Cell physiology · 2012Article
- Francisella tularensis type A strains cause the rapid encystment of Acanthamoeba castellanii and survive in amoebal cysts for three weeks postinfection.Applied and environmental microbiology · 2009Article
- A heterozygous mutation of beta-actin associated with neutrophil dysfunction and recurrent infection.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Arp2/3 complex from Acanthamoeba binds profilin and cross-links actin filaments.Molecular biology of the cell · 1998Article
- A phage display technique for a fast, sensitive, and systematic investigation of protein-protein interactions.Journal of protein chemistry · 1997Article
- ADP-ribosylation of actin by Clostridium perfringens iota toxin and turkey erythrocyte ADP-ribosyltransferase A: effects on profilin-regulated nucleotide exchange and ATPase activity.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae.The Journal of cell biology · 1996Article
- Structural connectivity in actin: effect of C-terminal modifications on the properties of actin.Biophysical journal · 1994Article
- X-ray structures of isoforms of the actin-binding protein profilin that differ in their affinity for phosphatidylinositol phosphates.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Mapping actin surfaces required for functional interactions in vivo.The Journal of cell biology · 1994Article
- Mutational analysis of yeast profilin.Molecular and cellular biology · 1993Article
- Three-dimensional solution structure of Acanthamoeba profilin-I.The Journal of cell biology · 1993Article
- Molecular genetics of actin function.The Biochemical journal · 1993Review
- The EGF receptor is an actin-binding protein.The Journal of cell biology · 1992Article
- The control of actin nucleotide exchange by thymosin beta 4 and profilin. A potential regulatory mechanism for actin polymerization in cells.Molecular biology of the cell · 1992Article
- Effects of profilin and profilactin on actin structure and function in living cells.The Journal of cell biology · 1992Article
- The interaction of actin with thymosin beta 4.Journal of muscle research and cell motility · 1992Article
- Are the conserved sequences in segment 1 of gelsolin important for binding actin?The Journal of cell biology · 1992Article
- Characterization of actin filament severing by actophorin from Acanthamoeba castellanii.The Journal of cell biology · 1991Article
Corrections and comments
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3 authors.
Funding
Abstract
Acanthamoeba profilin was cross-linked to actin via a zero-length isopeptide bond using carbodiimide. The covalently linked 1:1 complex was purified and treated with cyanogen bromide. This cleaves actin into small cyanogen bromide (CNBr) peptides and leaves the profilin intact owing to its lack of methionine. Profilin with one covalently attached actin CNBr peptide was purified by gel filtration followed by gel electrophoresis and electroblotting on polybase-coated glass-fiber membranes. Since the NH2 terminus of profilin is blocked, Edman degradation gave only the sequence of the conjugated actin CNBr fragment beginning with Trp-356. The profilin-actin CNBr peptide conjugate was digested further with trypsin and the cross-linked peptide identified by comparison with the tryptic peptide pattern obtained from carbodiimide-treated profilin. Amino-acid sequence analysis of the cross-linked tryptic peptides produced two residues at each cycle. Their order corresponds to actin starting at Trp-356 and profilin starting at Ala-94. From the absence of the phenylthiohydantoin-amino acid residues in specific cycles, we conclude that actin Glu-364 is linked to Lys-115 in profilin. Experiments with the isoforms of profilin I and profilin II gave identical results. The cross-linked region in profilin is homologous with sequences in the larger actin filament capping proteins fragmin and gelsolin.
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