ArticleClinical and vaccine immunology : CVI2015
Immunoproteomic Identification of In Vivo-Produced Propionibacterium acnes Proteins in a Rabbit Biofilm Infection Model.
Article in Clinical and vaccine immunology : CVI, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Does fluid irrigation in arthroscopic shoulder biopsy affect sensitivity ofJSES international · 2025Article
- Cutibacterium acnes biofilm formation is influenced by bone microenvironment, implant surfaces and bacterial internalization.BMC microbiology · 2024Article
- Proteomic and transcriptomic analyses ofAIMS microbiology · 2024Article
- Review
- Secretory Proteases of the Human Skin Microbiome.Infection and immunity · 2022Article
- Improve Integration of In Vitro Biofilm Body of Knowledge to Support Clinical Breakthroughs in Surgical Site Infection.Journal of the American Academy of Orthopaedic Surgeons. Global research & reviews · 2021Review
- A Janus-Faced Bacterium: Host-Beneficial and -Detrimental Roles ofFrontiers in microbiology · 2021Review
- The Role ofBiomedicines · 2020Article
- Cutibacterium (formerly Propionibacterium) acnes clavicular infection.Journal of bone and joint infection · 2019Article
- Host-microbiome interactions and recent progress into understanding the biology of acne vulgaris.Microbiome · 2018Review
- Clinical and Biological Features of Cutibacterium (Formerly Propionibacterium)Clinical microbiology reviews · 2018Review
- Perioperative Antibiotic Prophylaxis Has No Effect on Time to Positivity and Proportion of Positive Samples: a Cohort Study of 64 Cutibacterium acnes Bone and Joint Infections.Journal of clinical microbiology · 2018Article
- Propionibacterium avidum: A Virulent Pathogen Causing Hip Periprosthetic Joint Infection.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- Article
- Overcoming Gene-Delivery Hurdles: Physiological Considerations for Nonviral Vectors.Trends in biotechnology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Propionibacterium acnes is well-known as a human skin commensal but can also act as an invasive pathogen causing implant-associated infections. In order to resolve these types of P. acnes infections, the implants must be removed, due to the presence of an established biofilm that is recalcitrant to antibiotic therapy. In order to identify those P. acnes proteins produced in vivo during a biofilm infection, we established a rabbit model of implant-associated infection with this pathogen. P. acnes biofilms were anaerobically grown on dextran beads that were then inoculated into the left tibias of rabbits. At 4 weeks postinoculation, P. acnes infection was confirmed by radiograph, histology, culture, and PCR. In vivo-produced and immunogenic P. acnes proteins were detected on Western blot using serum samples from rabbits infected with P. acnes after these bacterial proteins were separated by two-dimensional gel electrophoresis. Those proteins that bound host antibodies were then isolated and identified by tandem mass spectrometry. Radiographs and histology demonstrated a disruption in the normal bone architecture and adherent biofilm communities in those animals with confirmed infections. A total of 24 immunogenic proteins were identified; 13 of these proteins were upregulated in both planktonic and biofilm modes, including an ABC transporter protein. We successfully adapted a rabbit model of implant-associated infection for P. acnes to identify P. acnes proteins produced during a chronic biofilm-mediated infection. Further studies are needed to evaluate the potential of these proteins for either a diagnostic test or a vaccine to prevent biofilm infections caused by P. acnes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.