ArticleMolecular biology of the cell2015
PKCι interacts with Rab14 and modulates epithelial barrier function through regulation of claudin-2 levels.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Regulation of natural killer cell infiltration and tumor progression by CMTM6 in gastric cancer.BMC cancer · 2026Article
- Rab14 promotes Parkin-mediated mitophagy.Molecular biology of the cell · 2025Article
- P62/SQSTM1 binds with claudin-2 to target for selective autophagy in stressed intestinal epithelium.Communications biology · 2023Article
- Rab22a regulates the establishment of epithelial polarity.Small GTPases · 2021Article
- Roles of Interaction between CCN2 and Rab14 in Aggrecan Production by Chondrocytes.International journal of molecular sciences · 2020Article
- PX Domain-Containing Kinesin KIF16B and Microtubule-Dependent Intracellular Movements.The Journal of membrane biology · 2020Review
- Claudin-2: Roles beyond Permeability Functions.International journal of molecular sciences · 2019Review
- An emerging role for IQGAP1 in tight junction control.Small GTPases · 2018Review
- The Endosomal Protein Endotubin Is Required for Enterocyte Differentiation.Cellular and molecular gastroenterology and hepatology · 2018Article
- Rab14 specifies the apical membrane through Arf6-mediated regulation of lipid domains and Cdc42.Scientific reports · 2016Article
- Conditional knockout of polarity complex (atypical) PKCι reveals an anti-inflammatory function mediated by NF-κB.Molecular biology of the cell · 2016Article
- Regulation of endothelial and epithelial barrier functions by peptide hormones of the adrenomedullin family.Tissue barriers · 2016Review
- Zeta Inhibitory Peptide Disrupts Electrostatic Interactions That Maintain Atypical Protein Kinase C in Its Active Conformation on the Scaffold p62.The Journal of biological chemistry · 2015Article
- Multiple roles for keratin intermediate filaments in the regulation of epithelial barrier function and apico-basal polarity.Tissue barriersReview
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
PKCι is essential for the establishment of epithelial polarity and the normal assembly of tight junctions. We find that PKCι knockdown does not compromise the steady-state distribution of most tight junction proteins but results in increased transepithelial resistance (TER) and decreased paracellular permeability. Analysis of the levels of tight junction components demonstrates that claudin-2 protein levels are decreased. However, other tight junction proteins, such as claudin-1, ZO-1, and occludin, are unchanged. Incubation with an aPKC pseudosubstrate recapitulates the phenotype of PKCι knockdown, including increased TER and decreased levels of claudin-2. In addition, overexpression of PKCι results in increased claudin-2 levels. ELISA and coimmunoprecipitation show that the TGN/endosomal small GTPase Rab14 and PKCι interact directly. Immunolabeling shows that PKCι and Rab14 colocalize in both intracellular puncta and at the plasma membrane and that Rab14 expression is required for normal PKCι distribution in cysts in 3D culture. We showed previously that knockdown of Rab14 results in increased TER and decreased claudin-2. Our results suggest that Rab14 and aPKC interact to regulate trafficking of claudin-2 out of the lysosome-directed pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.